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Engineering the fragment crystallizable (Fc) region of human IgG1 multimers and monomers to fine-tune interactions with sialic acid-dependent receptors

Blundell, Pat, Le, NPL, Allen, J, Watanabe, Y and Pleass, Richard (2017) 'Engineering the fragment crystallizable (Fc) region of human IgG1 multimers and monomers to fine-tune interactions with sialic acid-dependent receptors'. Journal of Biological Chemistry, Vol 292, pp. 12994-13007.

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Abstract

Multimeric fragment crystallizable (Fc) regions and Fc-fusion proteins are actively being explored as biomimetic replacements for intravenous immunoglobulin (IVIG) therapy, which is deployed to manage many diseases and conditions but is expensive and not always efficient. The Fc region of human IgG1 (IgG1-Fc) can be engineered into multimeric structures (hexa-Fcs) that bind their cognate receptors with high avidity. The critical influence of the unique N-linked glycan attached at Asn-297 on the structure and function of IgG1-Fc is well documented; however, whether the N-linked glycan has a similarly critical role in multimeric, avidly binding Fcs is unknown. Hexa-Fc contains two N-linked sites, at Asn-77 (equivalent to Asn-297 in the Fc of IgG1) and Asn-236 (equivalent to Asn-563 in the tailpiece of IgM). We report here that glycosylation at Asn-297 is critical for interactions with Fc receptors and complement and that glycosylation at Asn-563 is essential for controlling multimerization. We also found that introduction of an additional fully occupied N-linked glycosylation site at the N-terminus at position 1 (equivalent to Asp-221 in the Fc of IgG1) dramatically enhances overall sialic acid content of the Fc multimers. Furthermore, replacement of Cys-575 in the IgM tailpiece of multimers resulted in monomers with enhanced sialic acid content and differential receptor-binding profiles. Thus, insertion of additional N-linked glycans into either the hinge or tailpiece of monomers or multimers leads to molecules with enhanced sialylation that may be suitable for managing inflammation or blocking pathogen invasion.

Item Type: Article
Subjects: QS Anatomy > QS 4 General works. Classify here works on regional anatomy
QU Biochemistry > Proteins. Amino Acids. Peptides > QU 55 Proteins
QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies
WB Practice of Medicine > Therapeutics > WB 340 Drug Administration
WH Hemic and Lymphatic Systems > Lymphatic System > WH 650 Reticuloendothelial system
Faculty: Department: Biological Sciences > Parasitology Department
Digital Object Identifer (DOI): https://doi.org/10.1074/jbc.M117.795047
Depositing User: Lynn Roberts-Maloney
Date Deposited: 02 Aug 2017 14:13
Last Modified: 29 Sep 2017 15:28
URI: http://archive.lstmed.ac.uk/id/eprint/7305

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