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Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis.

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Gómez-Baena, Guadalupe, Bennett, Richard J, Martínez-Rodríguez, Carmen, Wnęk, Małgorzata, Laing, Gavin, Hickey, Graeme, McLean, Lynn, Beynon, Robert J and Carrol, Enitan D (2017) 'Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis.'. Scientific reports, Vol 7, Issue 1, e7042.

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Abstract

Streptococcus pneumoniae is responsible for diseases causing major global public health problems, including meningitis, pneumonia and septicaemia. Despite recent advances in antimicrobial therapy, pneumococcal meningitis remains a life-threatening disease. Furthermore, long-term sequelae are a major concern for survivors. Hence, a better understanding of the processes occurring in the central nervous system is crucial to the development of more effective management strategies. We used mass spectrometry based quantitative proteomics to identify protein changes in cerebrospinal fluid from children with Streptococcus pneumoniae infection, compared with children admitted to hospital with bacterial meningitis symptoms but negative diagnosis. Samples were analysed, by label free proteomics, in two independent cohorts (cohort 1: cases (n = 8) and hospital controls (n = 4); cohort 2: cases (n = 8), hospital controls (n = 8)). Over 200 human proteins were differentially expressed in each cohort, of which 65% were common to both. Proteins involved in the immune response and exosome signalling were significantly enriched in the infected samples. For a subset of proteins derived from the proteome analysis, we corroborated the proteomics data in a third cohort (hospital controls (n = 15), healthy controls (n = 5), cases (n = 20)) by automated quantitative western blotting, with excellent agreement with our proteomics findings. Proteomics data are available via ProteomeXchange with identifier PXD004219.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 460 Genomics. Proteomics
QY Clinical Pathology > Diagnostic Tests > QY 250 Immunodiagnostic tests
WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 210 Streptococcal infections (General or not elsewhere classified)
WS Pediatrics > Diseases of Children and Adolescents > By System > WS 340 Nervous system
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1038/s41598-017-07127-6
SWORD Depositor: JISC Pubrouter
Depositing User: JISC Pubrouter
Date Deposited: 08 Sep 2017 11:12
Last Modified: 13 Apr 2018 09:01
URI: https://archive.lstmed.ac.uk/id/eprint/7481

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