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Functional Analysis of Phagocyte Activity in Whole Blood from HIV/Tuberculosis-Infected Individuals Using a Novel Flow Cytometry-Based Assay.

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Gupta-Wright, Ankur, Tembo, Dumizulu, Jambo, Kondwani C, Chimbayo, Elizabeth, Mvaya, Leonard, Caldwell, Shannon, Russell, David G and Mwandumba, Henry (2017) 'Functional Analysis of Phagocyte Activity in Whole Blood from HIV/Tuberculosis-Infected Individuals Using a Novel Flow Cytometry-Based Assay.'. Frontiers in immunology, Vol 8, p. 1222.

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Abstract

The accurate assessment of immune competence through ex vivo analysis is paramount to our understanding of those immune mechanisms that lead to protection or susceptibility against a broad range of human pathogens. We have developed a flow cytometry-based, whole blood phagocyte functional assay that utilizes the inflammatory inducer zymosan, coupled to OxyBURST-SE, a fluorescent reporter of phagosomal oxidase activity. The assay measures both phagocytic uptake and the superoxide burst in the phagocyte populations in whole blood. We utilized this assay to demonstrate impaired superoxide burst activity in the phagocytes of hospitalized HIV-positive patients with laboratory-confirmed tuberculosis. These data validate the use of the assay to assess the immune competence of patients in a clinical setting. The method is highly reproducible with minimal intraindividual variation and opens opportunities for the rapid assessment of cellular immune competence in peripheral blood in a disease setting.

Item Type: Article
Subjects: QW Microbiology and Immunology > Reference Works. General Immunology > QW 504 General works
QY Clinical Pathology > Blood. Blood Chemistry > QY 450 Blood chemistry
WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503 Acquired immunodeficiency syndrome. HIV infections
WF Respiratory System > Tuberculosis > WF 200 Tuberculosis (General)
WF Respiratory System > Tuberculosis > WF 215 Pathology
WH Hemic and Lymphatic Systems > WH 100 General works
WH Hemic and Lymphatic Systems > WH 20 Research (General)
Faculty: Department: Clinical Sciences & International Health > Malawi-Liverpool-Wellcome Programme (MLW)
Digital Object Identifer (DOI): https://doi.org/10.3389/fimmu.2017.01222
SWORD Depositor: JISC Pubrouter
Depositing User: Stacy Murtagh
Date Deposited: 31 Oct 2017 15:32
Last Modified: 31 Oct 2017 15:32
URI: https://archive.lstmed.ac.uk/id/eprint/7757

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