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Absence of Wolbachia endobacteria in the non-filariid nematodes Angiostrongylus cantonensis and A. costaricensis

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Foster, J. M., Kumar, S., Ford, Louise, Johnston, Kelly, Ben, R., Graeff-Teixeira, C. and Taylor, Mark (2008) 'Absence of Wolbachia endobacteria in the non-filariid nematodes Angiostrongylus cantonensis and A. costaricensis'. Parasites & Vectors, Vol 1, e31.

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Abstract

The majority of filarial nematodes harbour Wolbachia endobacteria, including the major pathogenic species in humans, Onchocerca volvulus, Brugia malayi and Wuchereria bancrofti. These obligate endosymbionts have never been demonstrated unequivocally in any non-filariid nematode. However, a recent report described the detection by PCR of Wolbachia in the metastrongylid nematode, Angiostrongylus cantonensis (rat lungworm), a leading cause of eosinophilic meningitis in humans. To address the intriguing possibility of Wolbachia infection in nematode species distinct from the Family Onchocercidae, we used both PCR and immunohistochemistry to screen samples of A. cantonensis and A. costaricensis for the presence of this endosymbiont. We were unable to detect Wolbachia in either species using these methodologies. In addition, bioinformatic and phylogenetic analyses of the Wolbachia gene sequences reported previously from A. cantonensis indicate that they most likely result from contamination with DNA from arthropods and filarial nematodes. This study demonstrates the need for caution in relying solely on PCR for identification of new endosymbiont strains from invertebrate DNA samples.

Item Type: Article
Additional Information: The electronic version of this article is the complete one and can be found online at: http://www.parasitesandvectors.com/content/1/1/31
Subjects: WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
QX Parasitology > Helminths. Annelida > QX 203 Nematoda
QX Parasitology > Helminths. Annelida > QX 301 Filarioidea
QX Parasitology > Arthropods > QX 460 Arthropods
QX Parasitology > Helminths. Annelida > QX 243 Strongyloidea
WC Communicable Diseases > Rickettsiaceae Infections. Chlamydiaceae Infections > WC 600 Rickettsiaceae infections. Chlamydiaceae infections. Tick-borne diseases
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1186/1756-3305-1-31
Depositing User: Mary Creegan
Date Deposited: 25 Jun 2010 11:11
Last Modified: 06 Feb 2018 13:00
URI: http://archive.lstmed.ac.uk/id/eprint/780

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