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Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA.

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Molina-Moya, Barbara, Gomgnimbou, Michel K, Spinasse, Lizania, Obasanya, Joshua, Oladimeji, Olanrewaju, Dacombe, Russell ORCID: https://orcid.org/0000-0002-6705-1537, Edwards, Thomas, Daragon, Xavier-Olessa, Lawson, Lovett, Abdurrahman, Saddiq T, Cuevas, Luis ORCID: https://orcid.org/0000-0002-6581-0587, Dominguez, Jose and Sola, Christophe (2018) 'Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA.'. PLoS Neglected Tropical Diseases, Vol 12, Issue 2, e0006242.

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Abstract

All State TB control programmes in Nigeria were requested to submit 25-50 smear-positive Ziehl-Neelsen (ZN) stained slides for screening during 2013-2014. DNA was extracted from 929 slides for spoligotyping and drug-resistance analysis using microbead-based flow-cytometry suspension arrays. Spoligotyping results were obtained for 549 (59.1%) of 929 samples. Lineage 4 Cameroon sublineage (L4.6.2) represented half of the patterns, Mycobacterium africanum (L5 and L6) represented one fifth of the patterns, and all other lineages, including other L4 sublineages, represented one third of the patterns. Sublineage L4.6.2 was mostly identified in the north of the country whereas L5 was mostly observed in the south and L6 was scattered. The spatial distribution of genotypes had genetic geographic gradients. We did not obtain results enabling the detection of drug-resistance mutations. We present the first national snapshot of the M. tuberculosis spoligotypes circulating in Nigeria based on ZN slides. Spoligotyping data can be obtained in a rapid and high-throughput manner with DNA extracted from ZN-stained slides, which may potentially improve our understanding of the genetic epidemiology of TB.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 450 General Works
QU Biochemistry > Genetics > QU 475 Genetic processes
WC Communicable Diseases > Infection. Bacterial Infections > Other Bacterial Infections. Zoonotic Bacterial Infections > WC 302 Actinomycetales infections. Mycobacterium infections
WF Respiratory System > Tuberculosis > WF 200 Tuberculosis (General)
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.1371/journal.pntd.0006242
SWORD Depositor: JISC Pubrouter
Depositing User: Stacy Murtagh
Date Deposited: 28 Feb 2018 14:35
Last Modified: 11 May 2018 08:30
URI: https://archive.lstmed.ac.uk/id/eprint/8284

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