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Genome evolution of Wolbachia strain wPip from the Culex pipiens group

Klasson, L., Walker, T., Sebaihia, M., Sanders, M. J., Quail, M. A., Lord, A., Sanders, S., Earl, J., O'Neill, S. L., Thomson, N., Sinkins, Steven P. and Parkhill, J. (2008) 'Genome evolution of Wolbachia strain wPip from the Culex pipiens group'. Molecular Biology and Evolution, Vol 25, Issue 9, pp. 1877-1887.

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Abstract

The obligate intracellular bacterium Wolbachia pipientis strain wPip induces cytoplasmic incompatibility (CI), patterns of crossing sterility, in the Culex pipiens group of mosquitoes. The complete sequence is presented of the 1.48-Mbp genome of wPip which encodes 1386 coding sequences (CDSs), representing the first genome sequence of a B-supergroup Wolbachia . Comparisons were made with the smaller genomes of Wolbachia strains wMel of Drosophila melanogaster , an A-supergroup Wolbachia that is also a CI inducer, and wBm, a mutualist of Brugia malayi nematodes that belongs to the D-supergroup of Wolbachia . Despite extensive gene order rearrangement, a core set of Wolbachia genes shared between the 3 genomes can be identified and contrasts with a flexible gene pool where rapid evolution has taken place. There are much more extensive prophage and ankyrin repeat encoding (ANK) gene components of the wPip genome compared with wMel and wBm, and both are likely to be of considerable importance in wPip biology. Five WO-B-like prophage regions are present and contain some genes that are identical or highly similar in multiple prophage copies, whereas other genes are unique, and it is likely that extensive recombination, duplication, and insertion have occurred between copies. A much larger number of genes encode ankyrin repeat (ANK) proteins in wPip, with 60 present compared with 23 in wMel, many of which are within or close to the prophage regions. It is likely that this pattern is partly a result of expansions in the wPip lineage, due for example to gene duplication, but their presence is in some cases more ancient. The wPip genome underlines the considerable evolutionary flexibility of Wolbachia , providing clear evidence for the rapid evolution of ANK-encoding genes and of prophage regions. This host- Wolbachia system, with its complex patterns of sterility induced between populations, now provides an excellent model for unraveling the molecular systems underlying host reproductive manipulation.

Item Type: Article
Uncontrolled Keywords: endosymbiont wolbachia mosquito cytoplasmic incompatibility prophage ankyrin iv secretion system induced cytoplasmic incompatibility quinquefasciatus diptera endosymbionts wolbachia bacterial endosymbionts wuchereria-bancrofti filarial nematodes sexual alterations ankyrin repeat crossing type
Subjects: QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
QX Parasitology > Insects. Other Parasites > QX 505 Diptera
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > QX 45 Host-parasite relations
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 850 Nematode infections (General)
WC Communicable Diseases > Rickettsiaceae Infections. Chlamydiaceae Infections > WC 600 Rickettsiaceae infections. Chlamydiaceae infections. Tick-borne diseases
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1093/molbev/msn133
Depositing User: Users 183 not found.
Date Deposited: 15 Jun 2010 10:19
Last Modified: 06 Feb 2018 13:00
URI: https://archive.lstmed.ac.uk/id/eprint/829

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