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Differential expression of fat body genes in Glossina morsitans morsitans following infection with Trypanosoma brucei brucei

Lehane, Mike, Gibson, W. and Lehane, Stella (2008) 'Differential expression of fat body genes in Glossina morsitans morsitans following infection with Trypanosoma brucei brucei'. International Journal for Parasitology, Vol 38, Issue 1, pp. 93-101.

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Abstract

To determine which fat body genes were differentially expressed following infection of Glossina morsitans morsitans with Trypanosoma brucei brucei we generated four suppression subtractive hybridisation (SSH) libraries. We obtained 52 unique gene fragments (SSH clones) of which 30 had a known orthologue at E - 05 or less. Overall the characteristics of the orthologues suggest: (i) that trypanosome infection has a considerable effect on metabolism in the tsetse fly; (ii) that self-cured flies are mounting an oxidative stress response; and (iii) that self-cured flies are displaying increased energy usage. The three most consistently differentially expressed genes were further analysed by gene knockdown (RNAi). Knockdown of Glossina transferrin transcripts, which are upregulated in self-cured flies compared with flies infected with trypanosomes, results in a significant increase in the number of trypanosome infections establishing in the fly midgut, suggesting transferrin plays a role in the protection of tsetse flies from trypanosome infection. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: glossina trypanosoma ssh immunity rnai iron-binding proteins immune-response subtractive hybridization drosophila-melanogaster sleeping sickness aedes-aegypti tsetse transferrin identification hemolymph
Subjects: QX Parasitology > Insects. Other Parasites > QX 505 Diptera
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 705 Trypanosomiasis
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.ijpara.2007.06.004
Depositing User: Users 183 not found.
Date Deposited: 10 Jun 2010 12:28
Last Modified: 06 Feb 2018 13:00
URI: http://archive.lstmed.ac.uk/id/eprint/838

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