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Evidence of insulin-dependent signalling mechanisms produced by Citrus sinensis (L.) Osbeck fruit peel in an insulin resistant diabetic animal model

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Sathiyabama, Rajiv Gandhi, Gopalsamy, Rajiv Gandhi, Denadai, Marina, Sridharan, Gurunagarajan, Gnanasekaran, Jothi, Ponnusamy, Sasikumar, Siqueira Quintans, Jullyana de Souza, Narain, Narendra, Cuevas, Luis ORCID: https://orcid.org/0000-0002-6581-0587, Coutinho, Henrique Douglas Melo, Ramos, Andreza Guedes Barbosa, Quintans-Júnior, Lucindo José and Gurgel, Ricardo Queiroz (2018) 'Evidence of insulin-dependent signalling mechanisms produced by Citrus sinensis (L.) Osbeck fruit peel in an insulin resistant diabetic animal model'. Food and Chemical Toxicology, Vol 116, Issue B, pp. 86-99.

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Abstract

Citrus sinensis (L.) Osbeck is extensively cultivated worldwide and one of the most consumed fruits in the world. We evaluated the therapeutic properties of the methanol extract from Citrus sinensis fruit peel (CSMe) in high-fat diet-fed streptozotocin-induced insulin-resistant diabetic rats. Body weight, food intake, and water consumption were analysed. Biochemical and molecular biologic indices, and the expression of insulin receptor-induced signalling molecules were assessed to identify possible mechanisms. In addition, we conducted histology of pancreatic and adipose tissues. UHPLC-MS/MS analysis showed the presence of 17 dietary phenolics at substantial concentrations. High-fat diet-fed streptozotocin-induced diabetic rats administered CSMe (50 and 100 mg/kg) had reduced fasting blood glucose (56.1% and 55.7%, respectively) and plasma insulin levels (22.9% and 32.7%, respectively) compared with untreated diabetic control rats. CSMe reversed the biochemical abnormalities in diabetic rats, showed cytoprotective activity, and increased the intensity of the positive immunoreactions for insulin in pancreatic islets. CSMe treatment increased the expression of PPARγ in the adipose tissue and signalling molecules GLUT4 and insulin receptor. Our data suggest that CSMe could optimize glucose uptake of adipose tissues through the insulin-dependent signalling cascade mechanism and it should be investigated in the management of individuals with type 2 diabetes mellitus. [Abstract copyright: Copyright © 2018. Published by Elsevier Ltd.]

Item Type: Article
Subjects: QU Biochemistry > Vitamins > QU 220 Other vitamins
QV Pharmacology > Local Anti-Infective Agents. Dyes. Tars > QV 220 Local anti-infective agents. Disinfectants
WK Endocrine System > WK 810 Diabetes mellitus
Faculty: Department: Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.1016/j.fct.2018.03.050
SWORD Depositor: JISC Pubrouter
Depositing User: Stacy Murtagh
Date Deposited: 20 Apr 2018 15:39
Last Modified: 12 Sep 2018 09:49
URI: http://archive.lstmed.ac.uk/id/eprint/8488

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