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longevity of duodenal and peripheral T-cell and humoral responses to live-attenuated Salmonella Typhy strain Ty21a or Longevity of duodenal T-Cell Responses to TY21a

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Pennington, Shaun ORCID: https://orcid.org/0000-0002-7160-6275, Ferreira, Daniela ORCID: https://orcid.org/0000-0001-7730-9477, Reiné, Jesús, Nyarinda, Sheil, Thompson, Ameeka, Hancock, Carole, Wright, Angela, Gordon, Stephen ORCID: https://orcid.org/0000-0001-6576-1116 and Gordon, Melita (2018) 'longevity of duodenal and peripheral T-cell and humoral responses to live-attenuated Salmonella Typhy strain Ty21a or Longevity of duodenal T-Cell Responses to TY21a'. Vaccine, Vol 36, Issue 31, pp. 4725-4733.

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Abstract

Background
We have previously demonstrated that polyfunctional Ty21a-responsive CD4+ and CD8+ T cells are generated at the duodenal mucosa 18 days following vaccination with live-attenuated S. Typhi (Ty21a). The longevity of cellular responses has been assessed in peripheral blood, but persistence of duodenal responses is unknown.

Methods
We vaccinated eight healthy adults with Ty21a. Peripheral blood and duodenal samples were acquired after a median of 1.5 years (ranging from 1.1 to 3.7 years) following vaccination. Cellular responses were assessed in peripheral blood and at the duodenal mucosa by flow cytometry. Levels of IgG and IgA were also assessed in peripheral blood by enzyme-linked immunosorbent assay.

Results
No T-cell responses were observed at the duodenal mucosa, but CD4+ T-cell responses to Ty21a and FliC were observed in peripheral blood. Peripheral anti-lipopolysaccharide IgG and IgA responses were also observed. Early immunoglobulin responses were not associated with the persistence of long-term cellular immune responses.

Conclusions
Early T-cell responses which we have previously observed at the duodenal mucosa 18 days following oral vaccination with Ty21a could not be detected at a median of 1.5 years. Peripheral responses were observed at this time. Immunoglobulin responses observed shortly after vaccination were not associated with cellular immune responses at 1.5 years, suggesting that the persistence of cellular immunity is not associated with the strength of the initial humoral response to vaccination.

Item Type: Article
Subjects: QW Microbiology and Immunology > Bacteria > QW 138 Enterobacteriaceae
QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 573 Antigens
QY Clinical Pathology > Diagnostic Tests > QY 130 Gastric and duodenal contents
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.1016/j.vaccine.2018.05.114
Depositing User: Stacy Murtagh
Date Deposited: 06 Jul 2018 13:57
Last Modified: 06 Jul 2018 13:57
URI: http://archive.lstmed.ac.uk/id/eprint/8868

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