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Development and application of a simple colorimetric assay reveals widespread distribution of sodium channel mutations in Thai populations of Aedes aegypti

Rajatileka, Shavanthi, Black, W. C., Saavedra-Rodriguez, K., Trongtokit, Y., Apiwathnasorn, C., McCall, Philip ORCID: https://orcid.org/0000-0002-0007-3985 and Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247 (2008) 'Development and application of a simple colorimetric assay reveals widespread distribution of sodium channel mutations in Thai populations of Aedes aegypti'. Acta Tropica, Vol 108, Issue 1, pp. 54-57.

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Abstract

Dengue fever and its more serious complications dengue haemorrhagic fever and dengue shock syndrome are growing public health problems in tropical and subtropical countries. In the absence of a vaccine, most dengue control programmes rely heavily on the use of insecticides to target the Aedes mosquito vectors. As a limited number of insecticides are routinely used in control, monitoring for the presence of resistance is an essential component of dengue prevention programmes. The pyrethroid insecticides target the voltage-gated sodium channel on the insects' neurons. Substitutions at residue 1016 of this protein have been associated with pyrethroid and DDT resistance in Aedes aegypti populations from Latin America and Asia. Here we report on the development of a simple colorimetric assay to detect these mutations in individual mosquitoes. Evaluation of this diagnostic assay on 180 Ae. aegypti individuals from Thailand revealed the presence of high frequencies of the Val1016Gly mutation throughout the country. The assay requires no specialised equipment and will enable monitoring for insecticide resistance associated alleles to be routinely incorporated into dengue surveillance operations. (C) 2008 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: kdr insecticide resistance dengue aedes aegypti knockdown resistance kdr anopheles-gambiae pyrethroid resistance insecticide venezuela gene
Subjects: WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases
WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
WC Communicable Diseases > Virus Diseases > Infectious Mononucleosis. Arbovirus Infections > WC 528 Dengue
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
QX Parasitology > Insects. Other Parasites > QX 525 Aedes
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.actatropica.2008.08.004
Depositing User: Users 183 not found.
Date Deposited: 05 Jul 2010 10:31
Last Modified: 06 Feb 2018 13:00
URI: http://archive.lstmed.ac.uk/id/eprint/900

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