Evaluation of an ELISA to assess the intensity of Fasciola hepatica infection in cattle

Salimi-Bejestani, M. R., Cripps, P. and Williams, Diana J.L. (2008) 'Evaluation of an ELISA to assess the intensity of Fasciola hepatica infection in cattle'. Veterinary Record, Vol 162, Issue 4, pp. 109-111.

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Abstract

An ELISA with a diagnostic sensitivity of 98 per cent and specificity of 96 per cent was evaluated as a means of assessing the intensity of Fasciola hepatica infection in cattle. A total of 294 blood samples were collected from infected cattle at a local abattoir, and the level of infection in each animal was assessed on the basis of the extent of liver pathology and the presence of flukes; 120 blood samples were also collected from uninfected cattle kept on a farm known to be free of F hepatica. The results indicated that there was a significant correlation (P<0.001) between the ELISA values and the intensity of infection. Values between 15 and 28 per cent of a positive control sample indicated a low intensity of infection, values between 28 and 50 per cent indicated a medium intensity of infection and values above 50 per cent indicated a high intensity of infection.

Item Type:	Article
Uncontrolled Keywords:	linked-immunosorbent-assay liver fluke antibody resistance calves sheep diagnosis antigens england wales
Subjects:	QX Parasitology > QX 20 Research (General) QX Parasitology > Helminths. Annelida > QX 365 Fasciola
Faculty: Department:	Groups (2002 - 2012) > Veterinary Parasitology Group (2002-2008)
Depositing User:	Ms Julia Martin
Date Deposited:	01 Jul 2010 15:21
Last Modified:	06 Feb 2018 13:00
URI:	https://archive.lstmed.ac.uk/id/eprint/910

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