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Genomic analysis of detoxification genes in the mosquito Aedes aegypti

Strode, Clare, Wondji, Charles ORCID: https://orcid.org/0000-0003-0791-3673, David, Jean-Philippe, Hawkes, Nicola J., Lumjuan, Nongkran, Nelson, D. R., Drane, D. R., Karunaratne, S. H. P. P., Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173, Black, W. C. and Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247 (2008) 'Genomic analysis of detoxification genes in the mosquito Aedes aegypti'. Insect Biochemistry and Molecular Biology, Vol 38, Issue 1, pp. 113-123.

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Abstract

Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/ cholinesterase families in Ae. aegypti. This gene count represents an increase of 58% and 36% compared with the fruitfly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae, respectively. The expansion is not uniform within the gene families. Secure orthologs can be found across the insect species for enzymes that have presumed or proven biosynthetic or housekeeping roles. In contrast, subsets of these gene families that are associated with general xenobiotic detoxification, in particular the CYP6, CYP9 and alpha esterase families, have expanded in Ae. aegypti. In order to identify detoxification genes associated with resistance to insecticides we constructed an array containing unique oligonucleotide p robes for these genes and compared their expression level in insecticide resistant and susceptible strains. Several candidate genes were identified with the majority belonging to two gene families, the CYP9 P450s and the Epsilon GSTs. This 'Ae. aegypti Detox Chip' will facilitate the implementation of insecticide resistance management strategies for arboviral control programmes. (c) 2007 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: aedes aegypti p450 glutathione transferases carboxylesterases detox chip microarray pyrethroid-resistant strain insecticide resistance drosophila-melanogaster anopheles-gambiae culex-quinquefasciatus malaria vector p450 reductase house-fly expression cytochrome-p450
Subjects: QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics.
QW Microbiology and Immunology > Immunity by Type > QW 553 Maternally acquired immunity
QW Microbiology and Immunology > Immunity by Type > QW 551 Acquired immunity. Artificial immunity
QW Microbiology and Immunology > Immunity by Type > QW 545 Autoimmunity
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
QX Parasitology > Insects. Other Parasites > QX 505 Diptera
WC Communicable Diseases > Virus Diseases > Infectious Mononucleosis. Arbovirus Infections > WC 528 Dengue
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
QX Parasitology > Insects. Other Parasites > QX 525 Aedes
QW Microbiology and Immunology > Immunity by Type > QW 541 Natural immunity. Immunogenetics
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.ibmb.2007.09.007
Depositing User: Users 183 not found.
Date Deposited: 15 Jun 2010 11:30
Last Modified: 06 Feb 2018 13:00
URI: https://archive.lstmed.ac.uk/id/eprint/927

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