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Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR

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Lunde, Tracy Munthali, Roberts, Adam ORCID: https://orcid.org/0000-0002-0760-3088 and Al-Haroni, Mohammed (2018) 'Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR'. Journal of Oral Microbiology, Vol 11, Issue 1, p. 1552060.

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Abstract

Background: Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these elements.

Objectives and Design: In this study, the CN and CR of Tn916-Tn1545 elements in oral streptococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the influence of tetracycline on the CR of Tn916-Tn1545 elements.

Results: The ddPCR assay designed in this study is a reliable way to rapidly determine CN and CR of Tn916-Tn1545 elements.

Conclusions: Our data also suggest that Tn916-Tn1545 elements are generally stable without selective pressure in the clinical oral Streptococcus strains investigated in this study.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QU Biochemistry > Genetics > QU 475 Genetic processes
QU Biochemistry > Genetics > QU 550 Genetic techniques. PCR. Chromosome mapping
QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified.
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1080/20002297.2018.1552060
Depositing User: Lynn Roberts-Maloney
Date Deposited: 13 Dec 2018 15:33
Last Modified: 06 Sep 2019 09:34
URI: https://archive.lstmed.ac.uk/id/eprint/9797

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