Independent lineages of highly sulfadoxine-resistant Plasmodium falciparum haplotypes, Eastern Africa

Taylor, Steve M., Antonia, Alejandro L., Harrington, Whitney E., Goheen, Morgan M., Mwapasa, Victor, Chaluluka, Ebbie, Fried, Michal, Kabyemela, Edward, Madanitsa, Mwayi, Khairallah, Carole, Kalilani-Phiri, Linda, Tshefu, Antoinette K., Rogerson, Stephen J., terKuile, Feiko ORCID: https://orcid.org/0000-0003-3663-5617, Duffy, Patrick E. and Meshnick, Steven R. (2014) 'Independent lineages of highly sulfadoxine-resistant Plasmodium falciparum haplotypes, Eastern Africa'. Emerging Infectious Diseases, Vol 20, Issue 7, pp. 1140-1148.

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Abstract

Sulfadoxine-resistant Plasmodium falciparum undermines malaria prevention with sulfadoxine/pyrimethamine. Parasites with a highly resistant mutant dihydropteroate synthase (dhps) haplotype have recently emerged in eastern Africa; they negated preventive benefits of sulfadoxine/pyrimethamine, and might exacerbate placental malaria. We explored emerging lineages of dhps mutant haplotypes in Malawi, the Democratic Republic of the Congo, and Tanzania by using analyses of genetic microsatellites flanking the dhps locus. In Malawi, a triple-mutant dhps SGEG (mutant amino acids are underlined) haplotype emerged in 2010 that was closely related to pre-existing double-mutant SGEA haplotypes, suggesting local origination in Malawi. When we compared mutant strains with parasites from the Democratic Republic of the Congo and Tanzania by multiple independent analyses, we found that SGEG parasites were partitioned into separate lineages by country. These findings support a model of local origination of SGEG dhps haplotypes, rather than geographic diffusion, and have implications for investigations of emergence and effects of parasite drug resistance.

Item Type:	Article
Subjects:	QV Pharmacology > QV 38 Drug action. QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified. QX Parasitology > Protozoa > QX 135 Plasmodia WB Practice of Medicine > Therapeutics > WB 330 Drug therapy WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria WC Communicable Diseases > Tropical and Parasitic Diseases > WC 770 Therapy
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.3201/eid2007.131720
Depositing User:	Martin Chapman
Date Deposited:	27 Aug 2014 11:25
Last Modified:	31 May 2018 13:58
URI:	https://archive.lstmed.ac.uk/id/eprint/3791

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