Comparison of Two Genotyping Methods for Distinguishing Recrudescence from Reinfection in Antimalarial Drug Efficacy/Effectiveness Trials.

Fulakeza, Joseph R M, Banda, Rachel L, Lipenga, Trancizeo R, Terlouw, Anja ORCID: https://orcid.org/0000-0001-5327-8995, Nkhoma, Standwell and Hodel, EvaMaria ORCID: https://orcid.org/0000-0001-5821-1685 (2018) 'Comparison of Two Genotyping Methods for Distinguishing Recrudescence from Reinfection in Antimalarial Drug Efficacy/Effectiveness Trials.'. American Journal of Tropical Medicine and Hygiene, Vol 99, Issue 1, pp. 84-86.

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Abstract

Genotyping of allelic variants of merozoite surface proteins 1 and 2 ( and ), and the glutamate-rich protein is the gold standard for distinguishing reinfections from recrudescences in antimalarial drug trials. We compared performance of the recently developed 24-single-nucleotide polymorphism (SNP) Barcoding Assay against and genotyping in a cluster-randomized effectiveness trial of artemether-lumefantrine and dihydroartemisinin-piperaquine in Malawi. Rates of recrudescence and reinfection estimated by the two methods did not differ significantly (Fisher's exact test; = 0.887 and = 0.768, respectively). There was a strong agreement between the two methods in predicting treatment outcomes and resolving the genetic complexity of malaria infections in this setting. These results support the use of this SNP assay as an alternative method for correcting antimalarial efficacy/effectiveness data.

Item Type:	Article
Subjects:	QU Biochemistry > Genetics > QU 500 Genetic phenomena QU Biochemistry > Genetics > QU 550 Genetic techniques. PCR. Chromosome mapping QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 256 Antimalarials WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department:	Clinical Sciences & International Health > Malawi-Liverpool-Wellcome Programme (MLW)
Digital Object Identifer (DOI):	https://doi.org/10.4269/ajtmh.18-0002
Depositing User:	Stacy Murtagh
Date Deposited:	23 May 2018 10:17
Last Modified:	13 Sep 2019 13:11
URI:	https://archive.lstmed.ac.uk/id/eprint/8672

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