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Lynd, Amy 
ORCID: https://orcid.org/0000-0001-6054-0525, Gonahasa, Samuel, Staedke, Sarah G, Oruni, Ambrose, Maiteki-Sebuguzi, Catherine, Dorsey, Grant, Opigo, Jimmy, Yeka, Adoke, Katureebe, Agaba, Kyohere, Mary, Hemingway, Jane ORCID: https://orcid.org/0000-0002-3200-7173, Kamya, Moses R and Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497
(2019)
'LLIN Evaluation in Uganda Project (LLINEUP): a cross-sectional survey of species diversity and insecticide resistance in 48 districts of Uganda'. Parasites & Vectors.
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Abstract
AbstractBackground:Long‑lasting insecticidal nets (LLINs) are the principal tool for malaria control in Africa and are presently treated with a single class of insecticide; however, increasing levels of insecticide resistance threaten their success. In response to this threat nets have been developed that incorporate the synergist, piperonyl butoxide (PBO), which inhibits the activity of cytochrome P450s which is one main mechanisms of insecticide resistance, allowing resistance to pyrethroids to be reversed. However, data on the value and cost effectiveness of these nets is lacking. A large‑scale cluster randomised trial of conventional LLINs and PBO‑LLINs was conducted in Uganda in 104 health sub‑districts (HSDs) in 2017–2019. Prior to the mass distribution of LLINs, a baseline entomological survey was carried out, the results of which are reported herein. Ten households from each HSD were randomly selected for entomological sur‑veillance at baseline which included household mosquito collections.Results:Prior to LLIN distribution entomological collections were carried out in 1029 houses across the 104 HSDs. Anopheles gambiae (s.l.) was the principal vector in all but 9 of the 71 HSDs that yielded vector species. Molecular analysis found An. gambiae (s.s.) to be the predominant vector collected. Plasmodium falciparum was detected in 5.5% of An. gambiae (s.s.) and in 4.0% of An. funestus (s.s.) examined. Infection rates of other plasmodium species (P. vivax, P. ovale and P. malariae) were lower with infection rates of 1.2% and 1.7% for An. gambiae (s.s.) and An. funestus (s.s.), respectively. The knockdown resistance (kdr) mutation Vgsc‑L1014S was found at very high frequency in An. gambiae(s.s.) with the Vgsc‑L1014F mutation at low frequency and the wild‑type allele virtually absent. In An. arabiensis the wild‑type allele was predominant. The resistance‑associated alleles, Cyp4j5‑L43F and Coeae1d were found at moderate frequencies which varied across the study site. Vgsc‑N1575Y mutation was not found in any samples examined.Conclusions:No significant differences between planned intervention arms was observed in vector densities, sporo‑zoite infection rate or insecticide resistance marker frequency across the study site prior to the distribution of LLINs. Very high levels of kdr resistance were observed in all areas; however, the resistance‑associated markers Cyp4j5‑L43F and Coeae1d were found at varying frequencies across the study site which may have implications for the effective‑ness of standard LLINs
| Item Type: | Article |
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| Subjects: | QS Anatomy > QS 4 General works. Classify here works on regional anatomy QX Parasitology > Insects. Other Parasites > QX 500 Insects QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) WA Public Health > WA 30 Socioeconomic factors in public health (General) |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1186/s13071-019-3353-7 |
| Depositing User: | Stacy Murtagh |
| Date Deposited: | 28 Mar 2019 13:31 |
| Last Modified: | 16 Sep 2019 09:18 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/10522 |
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