LSTM Home > LSTM Research > LSTM Online Archive

PCR-based differentiation of Fasciola species (Trematoda : Fasciolidae), using primers based on RAPD-derived sequences

McGarry, J. W., Ortiz, P. L., Hodgkinson, Jane E., Goreish, I. and Williams, Diana J.L. (2007) 'PCR-based differentiation of Fasciola species (Trematoda : Fasciolidae), using primers based on RAPD-derived sequences'. Annals of Tropical Medicine and Parasitology, Vol 101, Issue 5, pp. 415-421.

Full text not available from this repository.


The zoonotic liver flukes Fasciola hepatica and F. gigantica co-exist in parts of Africa and Asia. The two species have similar life-cycles but different transmission characteristics. Although the identification of adult Fasciola to species level is traditionally based on differences in size and shape, recent studies have demonstrated this method to be unreliable. Species of Fasciola can be distinguished by staining and comparing the morpho-anatomy of the gut and ovaries or by iso-enzyme analysis but such approaches are time-consuming and require specialist skills. Two primer sets, based on RAPD-derived sequences from English F. hepatica and Ghanaian F. gigantica, can now be used, in two separate PCR, to distinguish F. hepatica from F. gigantica. When the PCR were used to investigate 10 flukes ( five from the U. K. and five from Peru) morpho- anatomically identified as F. hepatica and 10 ( five from Ghana and five from Sudan) morpho- anatomically identified as F. gigantica, all 20 flukes were correctly identified to species level. The PCR were validated using 175 flukes collected, over a 12- year period, from different countries and both cattle and sheep.

Item Type: Article
Uncontrolled Keywords: single juveniles hepatica gigantica assay DNA infection patterns
Subjects: QX Parasitology > Helminths. Annelida > QX 365 Fasciola
Faculty: Department: Groups (2002 - 2012) > Veterinary Parasitology Group (2002-2008)
Digital Object Identifer (DOI):
Related URLs:
Depositing User: Ms Julia Martin
Date Deposited: 16 Sep 2010 15:20
Last Modified: 06 Feb 2018 13:01


View details

Actions (login required)

Edit Item Edit Item