Brugia malayi Wolbachia hsp60 IgG antibody and isotype reactivity in different clinical groups infected or exposed to human bancroftian lymphatic filariasis

Suba, N., Shiny, C., Taylor, Mark ORCID: https://orcid.org/0000-0003-3396-9275 and Narayanan, R. B. (2007) 'Brugia malayi Wolbachia hsp60 IgG antibody and isotype reactivity in different clinical groups infected or exposed to human bancroftian lymphatic filariasis'. Experimental Parasitology, Vol 116, Issue 3, pp. 291-295.

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Abstract

Wolbachia, an endosymbiotic bacterium in filarial parasites, comes into contact with the host immune system upon parasite death. Here. we analyzed, total IgG and isotype antibody responses to Wolbachia hsp60 in individuals from an area endemic for Wuchereria bancrofti. Wolbachia derived hsp60 gene was cloned and the recombinant protein was used to determine the IgG and isotype reactivity by Western blotting and ELISA. All individuals from the endemic area generated antibody responses to Brugia malayi Wolbachia hsp60, which were elevated in the group with chronic pathology. Isotype analysis showed that, all clinical groups mounted IgGI-IgG4 responses with higher levels of B. malayi Wolbachia hsp60 specific IgGl observed in the sera of patients with chronic pathology compared to micro-filaraemics and endemic normals. These findings suggests that Wolbachia-derived hsp60 generates antibody responses in individuals infected or exposed to W bancrofti and an elevated IgG and IgG I reactivity is observed in people with filarial pathology. (c) 2007 Elsevier Inc. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	wolbachia wuchereria bancrofti brugio malavi heat shock protein 60 (hsp60) antibody isotype inflammation igq igg1, 2, 3, 4 heat-shock proteins wuchereria-bancrofti surface protein recognition subclasses nematodes parasite
Subjects:	QX Parasitology > Helminths. Annelida > QX 301 Filarioidea WC Communicable Diseases > Tropical and Parasitic Diseases > WC 850 Nematode infections (General) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.exppara.2006.12.006
Depositing User:	Ms Julia Martin
Date Deposited:	05 Oct 2010 12:06
Last Modified:	16 Sep 2019 09:00
URI:	https://archive.lstmed.ac.uk/id/eprint/1285

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