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Santos, A., Ribeiro, J. M. C., Lehane, Mike, Gontijo, N. F., Veloso, A. B., Sant'Anna, Mauricio R.V., Araujo, R. N., Grisard, E. C. and Pereira, M. H. (2007) 'The sialotranscriptome of the blood-sucking bug Triatoma brasiliensis (Hemiptera, Triatominae)'. Insect Biochemistry and Molecular Biology, Vol 37, Issue 7, pp. 702-712.
Full text not available from this repository.Abstract
Triatoma brasiliensis is the most important autochthon vector of Trypanosoma cruzi in Brazil, where it is widely distributed in the semiarid areas of the Northeast. In order to advance the knowledge of the salivary biomolecules of Triatominae, a salivary gland cDNA library of T brasiliensis was mass sequenced and analyzed. Polypeptides were sequenced by HPLC/Edman degradation experiments. Then 1712 cDNA sequences were obtained and grouped in 786 clusters. The housekeeping category had 24.4% and 17.8% of the clusters and sequences, respectively. The putatively secreted category contained 47.1% of the clusters and 68.2% of the sequences. Finally, 28.5% of the clusters, containing 14% of all sequences, were classified as unknown. The sialoma of T brasiliensis showed a high amount and great variety of different lipocalins (93.8% of secreted proteins). Remarkably, a great number of serine proteases that were not observed in previous blood-sucking sialotranscriptomes were found. Nine Kazal peptides were identified, among them one with high homology to the tabanid vasodilator vasotab, suggesting that the Triatoma vasodilator could be a Kazal protein. (c) 2007 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | saliva transcriptome hematophagy salivary proteins triatoma brasiliensis rhodnius prolixus stal salivary-gland nitric-oxide chagas-disease heme protein anticoagulant activity hematophagous insects platelet-aggregation molecular-cloning arthropod saliva |
| Subjects: | QX Parasitology > Insects. Other Parasites > QX 503 Hemiptera (e.g., Bedbugs) QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors |
| Faculty: Department: | Groups (2002 - 2012) > Vector Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.ibmb.2007.04.004 |
| Depositing User: | Ms Julia Martin |
| Date Deposited: | 07 Sep 2010 15:01 |
| Last Modified: | 06 Feb 2018 13:01 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1377 |
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