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A novel fluorescent probe for the detection of AmpC beta-lactamase and the application in screening beta-lactamase inhibitors

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Yu, Pan, Yang, Jia-Ning, Yan, Jin-Wu, Meng, Zhi-Zhong, Hong, David, Roberts, Adam P. ORCID: https://orcid.org/0000-0002-0760-3088, Ward, Stephen A. ORCID: https://orcid.org/0000-0003-2331-3192, Zhang, Lei and Li, Shan (2020) 'A novel fluorescent probe for the detection of AmpC beta-lactamase and the application in screening beta-lactamase inhibitors'. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol 234, e118257.

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Abstract

The rapid detection of β-lactamases (Blas) and effective screening of Bla inhibitors are critically important and urgent for solving antibiotic resistance and improving precision medicine. Here a novel fluorescent probe CDC-559 was designed and synthesized, which can be used for the selective and direct detection of AmpC Blas. More importantly, it can realize screening the Bla inhibitors with sulbactam sodium and tazobactam as model compounds, and the half-maximal inhibitory concentration are 0.279 μM and 0.053 μM, respectively. CDC-559 can be applied not only to examine the resistance of the strains, but also to categorize its mode of action specifically, which is consistent with the essential result of the Blas. The research suggests that CDC-559 probe features its tremendous potential in the rapid detection of AmpC Blas as well as the strains with AmpC-encoded gene, which is instructive in promoting better antibiotic stewardship practices and developments.

Item Type: Article
Subjects: QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified.
WB Practice of Medicine > Therapeutics > WB 330 Drug therapy
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1016/j.saa.2020.118257
Depositing User: Cathy Waldron
Date Deposited: 16 Mar 2020 13:48
Last Modified: 16 Apr 2020 15:30
URI: https://archive.lstmed.ac.uk/id/eprint/13984

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