Identification and typing of Cameroonian isolates of P-malariae using monoclonal antibodies against P-brasilianum

Zakeri, S., Lindergard, G., Davies, R. M., Boudin, C., Louis, F. and Hommel, Marcel (2006) 'Identification and typing of Cameroonian isolates of P-malariae using monoclonal antibodies against P-brasilianum'. Acta Tropica, Vol 99, Issue 1, pp. 97-101.

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Abstract

In the present study, monoclonal antibodies raised against Plasmodium brasilianum were used to demonstrate, for the first time, antigenic diversity in natural populations of Plasmodium malariae isolates and as diagnostic tool to detect low parasitaemia P. malariae infection. Seventeen McAbs reacting by indirect immunoflorescence antibody (IFA) assay with no other Plasmodium species than P. brasilianum, were shown to react with P. malariae and were used for typing 29 P. malariae isolates from hyperendemic areas in Yaounde and in three villages of South Cameroon with parasitaemia ranging from 0.01% to 1.8%. All 29 isolates were distinguished by their ability to react with certain antibodies and considered as representing different isolates of P malariae. One of these McAbs (No. 14) recognized P. malariae isolates to both in Yaounde and from Mengang but not in Edou or in Nkol Mvae, which may recognize a specific epitope that is less common in strains found in these villages and provide evidence of regional variation within the P. malariae parasites. The McAbs Nos. 16 and 17 were used to determine their usefulness as diagnostic tools for 30 suspected blood samples that were collected from patients with fever and it became clear that they could detect sub-microscopical infections of P. malariae. This study supports the concept of using of R brasilianum as a substitute for R malariae during immuno-diagnosis of malaria in endemic areas where PCR assay cannot be used for identification of the R malariae parasites. In addition our results for the first time provide evidence of considerable antigenic diversity of clinical R malariae isolates in Cameroon. (c) 2006 Elsevier B.V. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	p. malariae p. brasilianum monoclonal antibody antigenic diversity diagnosis plasmodium-brasilianum circumsporozoite protein nephrotic syndrome sporozoites parasite
Subjects:	QW Microbiology and Immunology > Immunity by Type > QW 568 Cellular immunity. Immunologic cytotoxicity. Immunocompetence. Immunologic factors (General) QX Parasitology > Protozoa > QX 135 Plasmodia WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department:	Groups (2002 - 2012) > Disease Control Strategy Group Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.actatropica.2006.08.004
Depositing User:	Martin Chapman
Date Deposited:	03 Mar 2011 12:46
Last Modified:	06 Feb 2018 13:02
URI:	https://archive.lstmed.ac.uk/id/eprint/1637

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