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Evaluating the diagnostic test accuracy of molecular xenomonitoring methods for characterising community burden of lymphatic filariasis

Pryce, Joseph and Reimer, Lisa ORCID: (2021) 'Evaluating the diagnostic test accuracy of molecular xenomonitoring methods for characterising community burden of lymphatic filariasis'. Clinical Infectious Diseases, Vol 72, Issue Supplement_3.

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Pryce Reimer - LF Molecular Xenomonitoring CID manuscript LReimer Feb 21.docx - Accepted Version
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Molecular xenomonitoring (MX), the detection of pathogen DNA in mosquitoes, is a recommended approach to support lymphatic filariasis (LF) elimination efforts. Potential roles of MX include detecting presence of LF in communities and quantifying progress towards elimination of the disease. However, the relationship between MX results and human prevalence is poorly understood.

:We conducted a systematic review and meta-analysis from all previously conducted studies that reported the prevalence of filarial DNA in wild-caught mosquitoes (MX rate) and the corresponding prevalence of microfilaria (mf) in humans. We calculated a pooled estimate of MX sensitivity for detecting positive communities at a range of mf prevalence values and mosquito sample sizes. We conducted a linear regression to evaluate the relationship between mf prevalence and MX rate.

We identified 24 studies comprising 144 study communities. MX had an overall sensitivity of 98.3% (95% CI 41.5, 99.9%) and identified 28 positive communities that were negative in the mf survey. Low sensitivity in some studies was attributed to small mosquito sample sizes (<1,000) and very low mf prevalence (<0.25%). Human mf prevalence and mass drug administration status accounted for approximately half of the variation in MX rate (R 2 = 0.49, p<0.001). Data from longitudinal studies showed that, within a given study area, there is a strong linear relationship between MX rate and mf prevalence (R 2 = 0.78, p < 0.001).

MX shows clear potential as tool for detecting communities where LF is present and as a predictor of human mf prevalence.

Item Type: Article
Subjects: W General Medicine. Health Professions > W 20.5 Biomedical research
WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):
Depositing User: Stacy Murtagh
Date Deposited: 29 Apr 2021 12:35
Last Modified: 10 Dec 2021 13:42


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