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Vanlandingham, D. L., Tsetsarkin, K., Hong, C., Klingler, K., McElroy, K. L., Lehane, Mike and Higgs, S. (2005) 'Development and characterization of a double subgenomic chikungunya virus infectious clone to express heterologous genes in Aedes aegypti mosqutioes'. Insect Biochemistry and Molecular Biology, Vol 35, Issue 10, pp. 1162-1170.
Full text not available from this repository.Abstract
Three full-length infectious cDNA clones based on the alphavirus chikungunya (CHIKV) were developed and characterized in vitro and in vivo. The full-length clone retained the viral phenotypes of CHIKV in both cell culture and in mosquitoes and should be a valuable tool for the study of virus interactions in an epidemiologically significant natural vector, Aedes aegypti. Two additional infectious clones were constructed that express green fluorescent protein (EGFP) in the midgut, salivary glands, and nervous tissue of Aedes aegypti mosquitoes following oral infection. The two constructs differed in the placement of the subgenomic promoter and the gene encoding EGFP. Viruses derived from the pCHIKic EGFP constructs (5' CHIKV EGFP and 3' CHIKV EGFP) expressed EGFP in 100% of the Ae. aegypti mosquitoes tested on days 7 and 14 post infection (p.i.). The 5' CHIKV EGFP disseminated to 90% of the salivary glands and nervous tissue by day 14 p.i. Dissemination rates of this new viral vector exceeds those of previous systems, thus expanding the repertoire and potential for gene expression studies on this important vector species. (c) 2005 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | mosquitoes aedes aegypti chikungunya virus green fluorescent protein gene expression yellow-fever mosquito green fluorescent protein recombinant sindbis virus per-os infection engineered resistance anopheles-gambiae transposable element transducing system malaria vector onyong-nyong |
| Subjects: | QX Parasitology > Insects. Other Parasites > QX 525 Aedes WC Communicable Diseases > Virus Diseases > General Virus Diseases > WC 500 Virus diseases (General or not elsewhere classified) |
| Faculty: Department: | Groups (2002 - 2012) > Vector Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.ibmb.2005.05.008 |
| Depositing User: | Ms Julia Martin |
| Date Deposited: | 30 Aug 2011 13:48 |
| Last Modified: | 06 Feb 2018 13:02 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1899 |
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