Njoroge, Harun, Van 't Hof, Arjen, Oruni, Ambrose, Pipini, Dimitra, Nagi, Sanjay, Lynd, Amy ORCID: https://orcid.org/0000-0001-6054-0525, Lucas, Eric ORCID: https://orcid.org/0000-0003-3892-1668, Tomlinson, Sean, Grau-Bové, Xavier, McDermott, Daniel, Wat'senga, Francis T., Manzambi, Emile Z., Agossa, Fiacre R., Mokuba, Arlette, Irish, Seth, Kabula, Bilali, Mbogo, Charles, Bargul, Joel, Paine, Mark ORCID: https://orcid.org/0000-0003-2061-7713, Weetman, David ORCID: https://orcid.org/0000-0002-5820-1388 and Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497 (2022) 'Identification of a rapidly‐spreading triple mutant for high‐level metabolic insecticide resistance in Anopheles gambiae provides a real‐time molecular diagnostic for antimalarial intervention deployment'. Molecular Ecology, Vol 31, Issue 16, pp. 4307-4318.
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Abstract
Studies of insecticide resistance provide insights into the capacity of populations to show rapid evolutionary responses to contemporary selection. Malaria control remains heavily dependent on pyrethroid insecticides, primarily in long lasting insecticidal nets (LLINs). Resistance in the major malaria vectors has increased in concert with the expansion of LLIN distributions. Identifying genetic mechanisms underlying high‐level resistance is crucial for the development and deployment of resistance‐breaking tools. Using the Anopheles gambiae 1000 genomes (Ag1000g) data we identified a very recent selective sweep in mosquitoes from Uganda which localized to a cluster of cytochrome P450 genes. Further interrogation revealed a haplotype involving a trio of mutations, a nonsynonymous point mutation in Cyp6p4 (I236M), an upstream insertion of a partial Zanzibar‐like transposable element (TE) and a duplication of the Cyp6aa1 gene. The mutations appear to have originated recently in An. gambiae from the Kenya‐Uganda border, with stepwise replacement of the double‐mutant (Zanzibar‐like TE and Cyp6p4‐236 M) with the triple‐mutant haplotype (including Cyp6aa1 duplication), which has spread into the Democratic Republic of Congo and Tanzania. The triple‐mutant haplotype is strongly associated with increased expression of genes able to metabolize pyrethroids and is strongly predictive of resistance to pyrethroids most notably deltamethrin. Importantly, there was increased mortality in mosquitoes carrying the triple‐mutation when exposed to nets cotreated with the synergist piperonyl butoxide (PBO). Frequencies of the triple‐mutant haplotype remain spatially variable within countries, suggesting an effective marker system to guide deployment decisions for limited supplies of PBO‐pyrethroid cotreated LLINs across African countries.
Item Type: | Article |
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Subjects: | QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 256 Antimalarials QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified. QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control |
Faculty: Department: | Biological Sciences > Vector Biology Department |
Digital Object Identifer (DOI): | https://doi.org/10.1111/mec.16591 |
SWORD Depositor: | JISC Pubrouter |
Depositing User: | JISC Pubrouter |
Date Deposited: | 17 Aug 2022 11:24 |
Last Modified: | 14 Jun 2023 10:00 |
URI: | https://archive.lstmed.ac.uk/id/eprint/20783 |
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