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Multiple insecticide resistance and first evidence of V410L kdr mutation in Aedes (Stegomyia) aegypti (Linnaeus) from Burkina Faso

Toé, Hyacinthe K., Zongo, Soumanaba, Guelbeogo, Moussa W., Kamgang, Basile, Viana, Mafalda, Tapsoba, Madou, Sanou, Antoine, Traoré, Alphonse, McCall, Philip ORCID: and Sagnon, N'Falé (2022) 'Multiple insecticide resistance and first evidence of V410L kdr mutation in Aedes (Stegomyia) aegypti (Linnaeus) from Burkina Faso'. Medical and Veterinary Entomology, Vol 36, Issue 3, pp. 309-319.

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The response to recent dengue outbreaks in Burkina Faso was insecticide‐based, despite poor knowledge of the vector population's susceptibility to the insecticides used. Here, we report on the susceptibility to the main insecticide classes and identify important underlying mechanisms in Aedes aegypti populations in Ouagadougou and Banfora, in 2019 and 2020. Wild Ae. aegypti were tested as adults in WHO bioassays and then screened in real time melting curve qPCR analyses to genotype the F1534C, V1016I, and V410L Aedes kdr mutations. Ae. aegypti showed moderate resistance to 0.1% bendiocarb (80–95% survival post‐exposure), 0.8% Malathion (60–100%), 0.21% pirimiphos‐methyl (75% – 97%), and high resistance to 0.03% deltamethrin (20–70%). PBO pre‐exposure partially restored pyrethroid susceptibility. Genotyping detected high frequency of 1534C allele (0.92) and moderate 1016I (0.1–0.32). The V410L mutation was detected in Burkina Faso for the first time (frequency 0.1–0.36). Mosquitoes surviving 4 h exposure to 0.03% deltamethrin had significantly higher frequencies of the F1534C mutation than dead mosquitoes (0.70 vs. 0.96, p < 0.0001) and mosquitoes surviving 2 ‐ 4 h exposure had a significantly reduced life span. Ae. aegypti from Burkina Faso are resistant to multiple insecticide classes with multiple mechanisms involved, demonstrating the essential role of insecticide resistance monitoring within national dengue control programmes.

Item Type: Article
Additional Information: This article also appears in: Special Issue: Sustainable Vector Biology Research Systems in Africa
Subjects: QU Biochemistry > Genetics > QU 500 Genetic phenomena
QX Parasitology > QX 20 Research (General)
QX Parasitology > Insects. Other Parasites > QX 525 Aedes
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):
SWORD Depositor: JISC Pubrouter
Depositing User: JISC Pubrouter
Date Deposited: 23 Aug 2022 13:50
Last Modified: 23 Aug 2022 13:50


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