Genetic markers associated with the widespread insecticide resistance in malaria vector Anopheles funestus populations across Tanzania

Odero, Joel O., Nambunga, Ismail H., Masalu, John P., Mkandawile, Gustav, Bwanary, Hamis, Hape, Emmanuel E., Njalambaha, Rukiyah M., Tungu, Patrick, Ngowo, Halfan S., Kaindoa, Emmanuel W., Mapua, Salum A., Kahamba, Najat F., Nelli, Luca, Wondji, Charles ORCID: https://orcid.org/0000-0003-0791-3673, Koekemoer, Lizette L., Weetman, David ORCID: https://orcid.org/0000-0002-5820-1388, Ferguson, Heather M., Baldini, Francesco and Okumu, Fredros O. (2024) 'Genetic markers associated with the widespread insecticide resistance in malaria vector Anopheles funestus populations across Tanzania'. Parasites & Vectors, Vol 17, Issue 1, e230.

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Abstract

Background: Anopheles funestus is a leading vector of malaria in most parts of East and Southern Africa, yet its ecology and responses to vector control remain poorly understood compared with other vectors such as Anopheles gambiae and Anopheles arabiensis. This study presents the first large-scale survey of the genetic and phenotypic expression of insecticide resistance in An. funestus populations in Tanzania.

Methods: We performed insecticide susceptibility bioassays on An. funestus mosquitoes in nine regions with moderate-to-high malaria prevalence in Tanzania, followed by genotyping for resistance-associated mutations (CYP6P9a, CYP6P9b, L119F-GSTe2) and structural variants (SV4.3 kb, SV6.5 kb). Generalized linear models were used to assess relationships between genetic markers and phenotypic resistance. An interactive R Shiny tool was created to visualize the data and support evidence-based interventions.

Results: Pyrethroid resistance was universal but reversible by piperonyl-butoxide (PBO). However, carbamate resistance was observed in only five of the nine districts, and dichloro-diphenyl-trichloroethane (DDT) resistance was found only in the Kilombero valley, south-eastern Tanzania. Conversely, there was universal susceptibility to the organophosphate pirimiphos-methyl in all sites. Genetic markers of resistance had distinct geographical patterns, with CYP6P9a-R and CYP6P9b-R alleles, and the SV6.5 kb structural variant absent or undetectable in the north-west but prevalent in all other sites, while SV4.3 kb was prevalent in the north-western and western regions but absent elsewhere. Emergent L119F-GSTe2, associated with deltamethrin resistance, was detected in heterozygous form in districts bordering Mozambique, Malawi and the Democratic Republic of Congo. The resistance landscape was most complex in western Tanzania, in Tanganyika district, where all five genetic markers were detected. There was a notable south-to-north spread of resistance genes, especially CYP6P9a-R, though this appears to be interrupted, possibly by the Rift Valley.

Conclusions: This study underscores the need to expand resistance monitoring to include An. funestus alongside other vector species, and to screen for both the genetic and phenotypic signatures of resistance. The findings can be visualized online via an interactive user interface and could inform data-driven decision-making for resistance management and vector control. Since this was the first large-scale survey of resistance in Tanzania’s An. funestus, we recommend regular updates with greater geographical and temporal coverage.

Item Type:	Article
Subjects:	QU Biochemistry > Genetics > QU 470 Genetic structures QX Parasitology > QX 20 Research (General) QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1186/s13071-024-06315-4
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	23 May 2024 11:44
Last Modified:	23 May 2024 11:44
URI:	https://archive.lstmed.ac.uk/id/eprint/24584

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