In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment

Morphew, Russell M., MacKintosh, Neil, Hart, Elizabeth H., Prescott, Mark, LaCourse, E. James ORCID: https://orcid.org/0000-0001-9261-7136 and Brophy, Peter M. (2014) 'In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment'. EuPA Open Proteomics, Vol 3, pp. 85-99.

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Abstract

The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ) is threatened by emerging resistance. F. hepatica excretory/secretory (ES) products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.

Item Type:	Article
Uncontrolled Keywords:	Proteomics; Biomarkers; Triclabendazole; Calreticulin
Subjects:	QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.5 DNA. QV Pharmacology > Drug Standardization. Pharmacognosy. Medicinal Plants > QV 744 Pharmaceutical chemistry QX Parasitology > Helminths. Annelida > QX 365 Fasciola WC Communicable Diseases > Tropical and Parasitic Diseases > WC 770 Therapy WC Communicable Diseases > Tropical and Parasitic Diseases > WC 805 Trematode infections (General)
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.euprot.2014.02.013
Depositing User:	Mary Creegan
Date Deposited:	09 May 2014 09:28
Last Modified:	06 Feb 2018 13:07
URI:	https://archive.lstmed.ac.uk/id/eprint/3685

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