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Rao, Ramakrishna U, Huang, Yuefang, Bockarie, Moses, Susapu, Melinda, Laney, Sandra J and Weil, Gary J (2009) 'A qPCR-based multiplex assay for the detection of Wuchereria bancrofti, Plasmodium falciparum and Plasmodium vivax DNA.'. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol 103, Issue 4, pp. 365-70.
Full text not available from this repository.Abstract
The purpose of this study was to develop real-time multiplex quantitative PCR (qPCR) assays for the simultaneous detection of Wuchereria bancrofti (Wb), Plasmodium falciparum (Pf) and P. vivax (Pv) in mosquitoes. We optimized the assays with purified DNA samples and then used these assays to test DNA samples isolated from Anopheles punctulatus mosquitoes collected in villages in Papua New Guinea where these infections are co-endemic. Singleplex assays detected Wb, Pf and Pv DNA in 32%, 19% and 15% of the mosquito pools, respectively, either alone or together with other parasites. Multiplex assay results agreed with singleplex results in most cases. Overall parasite DNA rates in mosquitoes, estimated by PoolScreen 2 software, for Wb, Pf and Pv were 4.9%, 2.7% and 2.1%, respectively. Parasite DNA rates were consistently higher in blood-fed mosquitoes than in host-seeking mosquitoes. Our results show that multiplex qPCR can be used to detect and estimate prevalence rates for multiple parasite species in arthropod vectors. We believe that multiplex molecular xenodiagnosis has great potential as a tool for non-invasively assessing the distribution and prevalence of vector-borne pathogens such as W. bancrofti and Plasmodium spp. in human populations and for assessing the impact of interventions aimed at controlling or eliminating these diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Wuchereria bancrofti Plasmodium falciparum Plasmodium vivax Mosquitoes Real-time PCR Xenomonitoring |
| Subjects: | WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Helminths. Annelida > QX 203 Nematoda QX Parasitology > Protozoa > QX 135 Plasmodia QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes WC Communicable Diseases > Tropical and Parasitic Diseases > WC 850 Nematode infections (General) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 885 Onchocerciasis |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.trstmh.2008.07.012 |
| Depositing User: | Users 67 not found. |
| Date Deposited: | 05 Jul 2010 11:46 |
| Last Modified: | 27 Sep 2018 10:35 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1063 |
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