Loss of introns along the evolutionary diversification pathway of snake venom disintegrins evidenced by sequence analysis of genomic DNA from Macrovipera lebetina transmediterranea and Echis ocellatus

Bazaa, A., Juarez, P., Marrakchi, N., Lasfer, Z. B., El Ayeb, M., Harrison, Robert, Calvete, J. J. and Sanz, L. (2007) 'Loss of introns along the evolutionary diversification pathway of snake venom disintegrins evidenced by sequence analysis of genomic DNA from Macrovipera lebetina transmediterranea and Echis ocellatus'. Journal of Molecular Evolution, Vol 64, Issue 2, pp. 261-271.

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Abstract

Analysis of cDNAs from Macrovipera lebetina transmediterranea (Mlt) and Echis ocellatus (Eo) venom gland libraries encoding disintegrins argued strongly for a common ancestry of the messengers of short disintegrins and those for precursors of dimeric disintegrin chains. We now report the sequence analysis of disintegrin-coding genes from these two vipers. Genomic DNAs for dimeric disintegrin subunits Ml_G1 and Ml_G2 (Mlt) and Eo_D3 ( Eo) contain single 1-kb introns exhibiting the 5'-GTAAG (donor)/3'-AG ( acceptor) consensus intron splicing signature. On the other hand, the short RTS-disintegrins M1_G3 ( Mlt) and Eo_RTS ( Eo) and the short RGD-disintegrin ocellatusin (Eo) are transcribed from intronless genomic DNA sequences, indicating that the evolutionary pathway leading to the emergence of short disintegrins involved the removal of all intronic sequences. The insertion position of the intron within Ml_G1, Ml_G2, and Eo_D3 is conserved in the genes for vertebrate ADAM ( A disintegrin and metalloproteinase) protein disintegrinlike domains and within the gene for the medium-size snake disintegrins halystatins 2 and 3. However, a comparative analysis of currently available disintegrin(-like) genes outlines the view that a minimization of both the gene organization and the protein structure underlies the evolution of the snake venom disintegrin family.

Item Type:	Article
Uncontrolled Keywords:	macrovipera lebetina transmediterranea echis ocellatus genomic DNA intron sequence intronless gene disintegrin evolution eristocophis-macmahoni venom heterodimeric disintegrin molecular-cloning functional-characterization phylogenetic analysis dimeric disintegrin angstrom resolution multigene families mass-spectrometry crystal-structure
Subjects:	QU Biochemistry > Genetics > QU 450 General Works WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1007/s00239-006-0161-4
Depositing User:	Ms Julia Martin
Date Deposited:	14 Oct 2010 15:00
Last Modified:	06 Feb 2018 13:01
URI:	https://archive.lstmed.ac.uk/id/eprint/1167

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