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Practical HPLC methods for the quantitative determination of common antimalarials in Africa

Bell, David J., Nyirongo, S. K., Molyneux, Malcolm E, Winstanley, P. A. and Ward, Stephen ORCID: https://orcid.org/0000-0003-2331-3192 (2007) 'Practical HPLC methods for the quantitative determination of common antimalarials in Africa'. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Vol 847, Issue 2, pp. 231-236.

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Abstract

This article describes high-performance liquid chromatographic assays for the quantification of sulfadoxine (SDX), pyrimethamine (PYM), chloroquine (CQ), amodiaquine, (AQ) and desethylamodiaquine (AQM) from whole blood. All four assays were set up and validated in Malawi using a common high-performance liquid chromatography platform and column and involved the use of simple mobile phase and extraction reagents. Calibration curves were linear (r(2) > 0.95) in the ranges 5-100 mu g/ml, 50-1000, 150-1500, 100-1000 and 100-1000 ng/ml for SDX, PYM, CQ, AQ and AQM, respectively. Intra-assay and inter-assay coefficients of variation were < 15% at 3 points spanning the concentration range and < 20% at the lower limit of quantification. The assays were specific with no interference from the other antimalarials described in this report. All four assays use liquid-liquid extraction, reversed-phase chromatography and UV detection and require between 50 and 200 mu l of blood. Because the assays share common instruments and reagents, they are cost-efficient and could be used to optimise antimalarial drug therapies in other resource poor settings. (c) 2006 Elsevier B.V. All rights reserved.

Item Type: Article
Additional Information: Bell (, Molyneux and Ward - LSTM authors.
Uncontrolled Keywords: sulfadoxine pyrimethamine chloroquine amodiaquine desethylamodiaquine high-performance liquid chromatography malaria acute falciparum-malaria filter-paper amodiaquine disposition chloroquine pharmacokinetics plasma
Subjects: QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 256 Antimalarials
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 770 Therapy
Faculty: Department: Groups (2002 - 2012) > Clinical Group
Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.jchromb.2006.10.020
Depositing User: Ms Julia Martin
Date Deposited: 16 Dec 2010 10:25
Last Modified: 17 Jul 2020 10:58
URI: https://archive.lstmed.ac.uk/id/eprint/1168

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