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Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization

Vontas, John, David, Jean-Philippe, Nikou, Dimitra, Hemingway, Janet ORCID:, Christophides, G. K., Louis, C. and Ranson, Hilary ORCID: (2007) 'Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization'. Insect Molecular Biology, Vol 16, Issue 3, pp. 315-324.

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A large scale microarray (20k MMC1) from the African malaria vector Anopheles gambiae was used to monitor gene expression in insecticide resistant and susceptible strains of the Asian mosquito Anopheles stephensi. Heterologous hybridization at slightly reduced stringency yielded -7000 significant signals. Thirty-six putative genes were differentially transcribed between the pyrethroid-resistant (DUB-R) and the susceptible (BEECH) strains. The expression profiles of selected transcripts were verified by real-time PCR. A gene putatively involved in the thickening of the adult cuticle showed the most striking up-regulation in DUB-R. A more specialized microarray containing 231 An. gambiae genes putatively involved in insecticide detoxification was used to further analyse classical insecticide resistance genes. Three glutathione S-transferase (GST) transcripts, one esterase and a cytochrome P450 were up-regulated in the resistant strain, while two peroxidases were down-regulated.

Item Type: Article
Uncontrolled Keywords: pyrethroid resistance heterologous hybridization detoxification chip malaria cytochrome-c-oxidase pyrethroid-resistant nilaparvata-lugens gene-expression drosophila-melanogaster susceptible strains glycolytic-enzymes brown planthopper malaria vector south-africa
Subjects: QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):
Depositing User: Users 183 not found.
Date Deposited: 19 Oct 2010 09:06
Last Modified: 30 Aug 2019 17:05


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