Mapping a quantitative trait locus (QTL) conferring pyrethroid resistance in the African malaria vector Anopheles funestus

Wondji, Charles ORCID: https://orcid.org/0000-0003-0791-3673, Morgan, John, Coetzee, M., Hunt, R. H., Steen, Keith ORCID: https://orcid.org/0000-0002-8933-8643, Black, W. C., Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 and Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247 (2007) 'Mapping a quantitative trait locus (QTL) conferring pyrethroid resistance in the African malaria vector Anopheles funestus'. Bmc Genomics, Vol 8.

Full text not available from this repository.

Abstract

Background: Pyrethroid resistance in Anopheles funestus populations has led to an increase in malaria transmission in southern Africa. Resistance has been attributed to elevated activities of cytochrome P450s but the molecular basis underlying this metabolic resistance is unknown. Microsatellite and SNP markers were used to construct a linkage map and to detect a quantitative trait locus ( QTL) associated with pyrethroid resistance in the FUMOZ-R strain of An. funestus from Mozambique.
Results: By genotyping 349 F-2 individuals from 11 independent families, a single major QTL, rp1, at the telomeric end of chromosome 2R was identified. The rp1 QTL appears to present a major effect since it accounts for more than 60% of the variance in susceptibility to permethrin. This QTL has a strong additive genetic effect with respect to susceptibility. Candidate genes associated with pyrethroid resistance in other species were physically mapped to An. funestus polytene chromosomes. This showed that rp1 is genetically linked to a cluster of CYP6 cytochrome P450 genes located on division 9 of chromosome 2R and confirmed earlier reports that pyrethroid resistance in this strain is not associated with target site mutations ( knockdown resistance).
Conclusion: We hypothesize that one or more of these CYP6 P450s clustered on chromosome 2R confers pyrethroid resistance in the FUMOZ-R strain of An. funestus.

Item Type:	Article
Additional Information:	The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2164/8/34
Uncontrolled Keywords:	insecticide resistance drosophila-melanogaster south-africa gambiae gene mosquito ddt map arabiensis culicidae
Subjects:	QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1186/1471-2164-8-34
Depositing User:	Ms Julia Martin
Date Deposited:	03 Sep 2010 08:22
Last Modified:	13 Sep 2019 15:47
URI:	https://archive.lstmed.ac.uk/id/eprint/1385

Statistics

View details

Actions (login required)

Edit Item