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Loraine, Jessica, Heinz, Eva 
ORCID: https://orcid.org/0000-0003-4413-3756, Soontarach, Roseathorn, Blackwell, Grace A, Stabler, Richard A, Voravuthikunchai, Supayang P, Srimanote, Potjanee, Kiratisin, Pattarachai, Thomson, Nicholas R and Taylor, Peter W
(2020)
'Genomic and Phenotypic Analyses of Acinetobacter baumannii Isolates From Three Tertiary Care Hospitals in Thailand'. Frontiers in Microbiology, Vol 11, Issue 548.
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Frontiers_accepted Eva Heinz March 2020.pdf - Accepted Version Available under License Creative Commons Attribution. Download (7MB) | Preview |
Abstract
Antibiotic resistant strains of Acinetobacter baumannii are responsible for a large and increasing burden of nosocomial infections in Thailand and other countries of Southeast Asia.
New approaches to their control and treatment are urgently needed and an attractive strategy is to remove the bacterial polysaccharide capsule, and thus the protection from the host’s immune system. To examine phylogenetic relationships, distribution of capsule chemotypes, acquired antibiotic resistance determinants, susceptibility to complement and other traits associated with
systemic infection, we sequenced 191 isolates from three tertiary referral hospitals in Thailand and used phenotypic assays to characterise key aspects of infectivity. Several distinct lineages
were circulating in three hospitals and the majority belonged to global clonal group 2 (GC2). Very high levels of resistance to carbapenems and other front-line antibiotics were found, as
were a number of widespread plasmid replicons. A high diversity of capsule genotypes was encountered, with only three of these (KL6, KL10 and KL47) showing more than 10% frequency. Almost 90% of GC2 isolates belonged to the most common capsule genotypes and
50 were fully resistant to the bactericidal action of human serum complement, most likely protected by their polysaccharide capsule, which represents a key determinant of virulence for systemic infection. Our study further highlights the importance to develop therapeutic strategies to remove the polysaccharide capsule from extensively drug-resistant A. baumanii during the course of systemic infection.
| Item Type: | Article |
|---|---|
| Subjects: | QW Microbiology and Immunology > Bacteria > QW 131 Gram-negative bacteria. QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified. QW Microbiology and Immunology > QW 52 Physiology and chemistry of microorganisms. Metabolism. WX Hospitals and Other Health Facilities > WX 20 Research (General) |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.3389/fmicb.2020.00548 |
| Depositing User: | Mel Finley |
| Date Deposited: | 07 Apr 2020 10:50 |
| Last Modified: | 01 May 2020 09:56 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/13942 |
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