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Simoni, Alekos, Hammond, Andrew M., beaghton, Andrea K.Beaghton, Galizi, Roberto, Taxiarchi, Chrysanthi, Kyrou, Kyros, Meacci, Dario, Gribble, Matthew, Morselli, Giulia, Burt, Austin, Nolan, Tony 
ORCID: https://orcid.org/0000-0002-2982-8333 and Crisanti, Andrea
(2020)
'A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae'. Nature Biotechnology, Vol 38, pp. 1054-1060.
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Male biased sex distorter gene drive_Final approved - Tony Nolan May 20.pdf - Accepted Version Available under License Creative Commons Attribution. Download (6MB) | Preview |
Abstract
Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10–14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control.
| Item Type: | Article |
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| Subjects: | QU Biochemistry > Genetics > QU 470 Genetic structures QX Parasitology > Insects. Other Parasites > QX 515 Anopheles WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1038/s41587-020-0508-1 |
| Depositing User: | Stacy Murtagh |
| Date Deposited: | 26 May 2020 12:53 |
| Last Modified: | 28 Sep 2020 09:03 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/14486 |
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