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Involvement of cytochrome P450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics

David, Jean-Philippe, Boyer, S., Mesneau, A., Ball, Amanda, Ranson, Hilary ORCID: and Dauphin-Villemant, C. (2006) 'Involvement of cytochrome P450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics'. Insect Biochemistry and Molecular Biology, Vol 36, Issue 5, pp. 410-420.

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The response of mosquito larvae to plant toxins found in their breeding sites was investigated by using Aedes aegypti larvae and toxic arborescent leaf litter as experimental models. The relation between larval tolerance to toxic leaf litter and cytochrome P450 monooxygenases (P450 s) was examined at the toxicological, biochemical and molecular levels. Larvae pre-exposed to toxic leaf litter show a higher tolerance to those xenobiotics together with a strong increase in P450 activity levels. This enzymatic response is both time- and dose-dependent. The use of degenerate primers from various P450 genes (CYPs) allowed us to isolate 16 new CYP genes belonging to CYP4, CYP6 and CYP9 families. Expression studies revealed a 2.3-fold over-expression of 1 CYP gene (CYP6ALI) after larval pre-exposure to toxic leaf litter, this gene being expressed at a high level in late larval and pupal stages and in fat bodies and midgut. The CYP6ALI protein has a high level of identity with other insect's CYPs involved in xenobiotic detoxification. The role of CYP genes in tolerance to natural xenobiotics and the importance of such adaptive responses in the capacity of mosquitoes to colonize new habitats and to develop insecticide resistance mechanisms are discussed. (c) 2006 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: toxic leaf litter cytochrome p450 monooxygenases (cyp) mosquito larvae plant xenobiotics induction decomposed leaf-litter in-house flies insecticide resistance anopheles-gambiae papilio-polyxenes degenerate primers molecular-cloning helicoverpa-zea manduca-sexta expression
Subjects: QU Biochemistry > QU 4 General works
QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics.
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 525 Aedes
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):
Depositing User: Sarah Lewis-Newton
Date Deposited: 25 Feb 2011 14:08
Last Modified: 17 Jul 2020 10:57


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