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Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship

Dillon, Rod, Ivens, A. C., Churcher, C., Holroyd, N., Quail, M. A., Rogers, Matthew E., Soares, M. B., Bonaldo, M. F., Casavant, T. L., Lehane, Mike and Bates, Paul (2006) 'Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship'. Genomics, Vol 88, Issue 6, pp. 831-840.

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An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, < 4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (pelitrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signating pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania. (c) 2006 Elsevier Inc. All rights reserved.

Item Type: Article
Uncontrolled Keywords: lutzomyia expressed sequence tag leishmania genomics immunity parasite midgut phlebotomus-papatasi diptera glossina-morsitans-morsitans mosquito anopheles-gambiae expressed sequence tags aedes-aegypti immune-response molecular characterization plasmodium-berghei midgut cells leishmania-mexicana
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QX Parasitology > QX 45 Host-parasite relations
QX Parasitology > Insects. Other Parasites > QX 505 Diptera
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):
Depositing User: Ms Julia Martin
Date Deposited: 13 Jan 2011 16:38
Last Modified: 17 Jul 2020 10:59


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