Tools
Tools
Juaez, P., Wagstaff, Simon 
ORCID: https://orcid.org/0000-0003-0577-5537, Oliver, J., Sanz, L., Harrison, Robert and Calvete, J. J.
(2006)
'Molecular cloning of disintegrin-like transcript BA-5A from a Bitis arietans venom gland cDNA library: A putative intermediate in the evolution of the long-chain disintegrin bitistatin'. Journal of Molecular Evolution, Vol 63, Issue 1, pp. 142-152.
Abstract
We report the cloning and sequence analysis of BA-5A from a venom gland cDNA library of the puff adder, Bitis arietans, that encodes a novel ECD-disintegrin-like domain. BA-5A is a unique PII disintegrin. It contains the 16 cysteine residues that are conserved in all known disintegrin-like domains of ADAM proteins and snake venom metalloproteinases but lacks the cysteine-rich domain. These features suggest that BA-5A may represent an intermediate in the evolutionary pathway of the long disintegrin bitistatin and that removal of the cysteine-rich domain and loss of the PIII-specific disulfide bond were separate events along the structural diversification pathway of disintegrins, the former predating the latter. The protein family composition of the Bitis arietans venom, as determined by combination of reversed-phase HPLC and proteomic analysis, was as follows: Zn2+-metalloproteinase (38.5%), serine proteinase (19.5%), disintegrin (17.8%), C-type lectin-like (13.2%), PLA(2) (4.3%), Kunitz-type inhibitor (4.1%), cystatin (1.7%), and unknown (0.9%). BA-5A could not be detected in the venom proteome of Bitis arietans. The occurrence of this very low-abundance (< 0.05%) or nonexpressed disintegrin transcript indicates a hitherto unrecognized structural diversity of this protein family. Whether BA-5A plays a physiological role or represents an orphan protein which could eventually evolve a role in the adaptation of snakes to changing ecological niches and prey habits deserves further investigation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | snake venomics venom proteome bitis arietans cdna cloning disintegrin evolution disulfide-bond pattern viper echis-carinatus crotalus-atrox venom c-type lectins snake-venom phylogenetic analysis platelet-aggregation macrovipera-lebetina dimeric disintegrin angstrom resolution |
| Subjects: | WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 400 General works WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles |
| Faculty: Department: | Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1007/s00239-005-0268-z |
| Depositing User: | Sarah Lewis-Newton |
| Date Deposited: | 08 Mar 2011 14:24 |
| Last Modified: | 17 Sep 2019 13:32 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1520 |
Statistics
Actions (login required)
 |
Edit Item |