Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays

Patterson, Edward I ORCID: https://orcid.org/0000-0003-3465-0848, Prince, Tessa, Anderson, Enyia, Casas Sanchez, Aitor ORCID: https://orcid.org/0000-0001-5237-1223, Smith, Shirley L, Cansado-Utrilla, Cintia, Solomon, Tom, Griffiths, Michael J, Acosta-Serrano, Alvaro ORCID: https://orcid.org/0000-0002-2576-7959, Turtle, Lance and Hughes, Grant ORCID: https://orcid.org/0000-0002-7567-7185 (2020) 'Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays'. Journal of Infectious Disease, Vol 222, Issue 9, pp. 1462-1467.

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Abstract

The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels.

Item Type:	Article
Subjects:	QV Pharmacology > Drug Standardization. Pharmacognosy. Medicinal Plants > QV 771 Standardization and evaluation of drugs QW Microbiology and Immunology > Viruses > QW 160 Viruses (General). Virology QY Clinical Pathology > QY 25 Laboratory techniques and procedure WA Public Health > WA 105 Epidemiology WC Communicable Diseases > Virus Diseases > Viral Respiratory Tract Infections. Respirovirus Infections > WC 505 Viral respiratory tract infections
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1093/infdis/jiaa507
Depositing User:	Samantha Sheldrake
Date Deposited:	03 Sep 2020 12:37
Last Modified:	29 Oct 2020 12:15
URI:	https://archive.lstmed.ac.uk/id/eprint/15446

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