Tools
Tools
Turner, Joseph 
ORCID: https://orcid.org/0000-0002-2185-5476, Langley, R. Stuart, Johnston, Kelly, Egerton, Gillian, Wanji, S. and Taylor, Mark ORCID: https://orcid.org/0000-0003-3396-9275
(2006)
'Wolbachia endosymbiotic bacteria of Brugia malayi mediate macrophage tolerance to TLR- and CD40-specific stimuli in a MyD88/TLR2-dependent manner'. Journal of Immunology, Vol 177, Issue 2, pp. 1240-1249.
Abstract
Lymphatic filarial nematodes are able to down-regulate parasite-specific and nonspecific responses of lymphocytes and APC. Lymphatic filariae are reliant on Wolbachia endosymbiotic bacteria for development and survival. We tested the hypothesis that repeated exposure to Wolbachia endosymbionts would drive macrophage tolerance in vitro and in vivo. We pre-exposed murine peritoneal-elicited macrophages to soluble extracts of Brugia malayi female worms (BMFE) before restimulating with BMFE or TLR agonists. BMFE tolerized macrophages (in terms of IFN-beta, IL-1 beta, IL-6, IL-12p40, and TNF-alpha inflammatory cytokine production) in a dose-dependent manner toward self, LPS, MyD88-dependent TLR2 or TLR9 ligands (peptidoglycan, triacyl lipopeptide, CpG DNA) and the MyD88-independent/TRIF-dependent TLR3 ligand, polyinosinic-polycytidylic acid. This was accompanied with down-regulation in surface expression of TLR4 and up-regulation of CD14, CD40, and TLR2. BMFE tolerance extended to CD40 activation in vitro and systemic inflammation following lethal challenge in an in vivo model of endotoxin shock. The mechanism of BMFE-mediated macrophage tolerance was dependent on MyD88 and TLR2 but not TLR4. Evidence that desensitization was driven by Wolbachia-specific ligands was determined by use of extracts from Wolbachia-depleted B. malayi, aposymbiotic filarial species, and a cell line stably infected with Wolbachia pipientis. Our data promote a role for Wolbachia in contributing toward the dysregulated and tolerized immunological phenotype that accompanies the majority of human filarial infections.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | growth-factor-beta filarial nematodes signaling pathway lymphatic filariasis immune-responses secreted product river-blindness surface protein cross-tolerance cutting edge |
| Subjects: | QX Parasitology > Helminths. Annelida > QX 203 Nematoda WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General) |
| Faculty: Department: | Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group |
| Depositing User: | Ms Julia Martin |
| Date Deposited: | 03 Mar 2011 17:01 |
| Last Modified: | 16 Sep 2019 09:00 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1618 |
Statistics
Actions (login required)
 |
Edit Item |