Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis

Molina-Moya, Barbara, Ciobanu, Nelly, Hernandez, Marta, Prat-Aymerich, Cristina, Crudu, Valeriu, Adams, Emily ORCID: https://orcid.org/0000-0002-0816-2835, Codreanu, Alexandru, Sloan, Derek, Cuevas, Luis ORCID: https://orcid.org/0000-0002-6581-0587 and Dominguez, Jose (2020) 'Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis'. Journal of clinical medicine, Vol 9, Issue 12, p. 4124.

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Abstract

Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, smear microscopy and X-Rays. Oral mucosa swabs collected on PrimeStore-MTM, stored at room temperature if tested within 30 days or at -20 °C if examined at a later time. RT-PCR was performed to detect M. tuberculosis DNA. Eighty patients had bacteriologically-confirmed TB, 34 had bacteriologically-negative TB (negative tests but abnormal X-rays) and 152 were considered not to have TB (not TB). Oral swabs RT-PCR were positive in 29/80 (36.3%) bacteriologically-confirmed, 9/34 (26.5%) bacteriologically-negative and 29/152 (19.1%) not TB. The yield varied among samples stored for less and more than 30 days (p = 0.013) from 61% (11/18) and 29% (18/62) among bacteriologically confirmed, and 30.8% (4/13) and 23.8% (5/21) among bacteriologically-negative participants. Among not TB patients, the specificity was 80.9% (123/152), being 78.3% (18/23) among samples stored less than 30 days and 81.4% (105/129) among samples stored for more than 30 days (p = 0.46). The detection of M. tuberculosis in oral mucosa samples is feasible, but storage conditions may affect the yield.

Item Type:	Article
Additional Information:	This article belongs to the Special Issue Recent Advances in Mycobacterial Research
Subjects:	QY Clinical Pathology > Diagnostic Tests > QY 120 Sputum WF Respiratory System > Tuberculosis > WF 200 Tuberculosis (General)
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.3390/jcm9124124
Depositing User:	Rachael O'Donoghue
Date Deposited:	04 Jan 2021 11:49
Last Modified:	04 Jan 2021 11:49
URI:	https://archive.lstmed.ac.uk/id/eprint/16435

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