Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes

Diaz, Alvaro, Kozak, Radoslaw P., MondragonShem, Karina, Williams, Chris, Rose, Clair ORCID: https://orcid.org/0000-0001-7782-5359, Perally, Samira, Caljon, Guy, Van Den Abbeele, Jan, Wongtrakul-Kish, Katherine, Gardner, Richard A., Spencer, Daniel, Lehane, Mike and Acosta-Serrano, Alvaro ORCID: https://orcid.org/0000-0002-2576-7959 (2021) 'Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes'. PLoS Neglected Tropical Diseases, Vol 15, Issue 2, e0009071.

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Abstract

African sleeping sickness is caused by Trypanosoma brucei, a parasite transmitted by the bite of a tsetse fly. Trypanosome infection induces a severe transcriptional downregulation of tsetse genes encoding for salivary proteins, which reduces its anti-hemostatic and anticlotting properties. To better understand trypanosome transmission and the possible role of glycans in insect bloodfeeding, we characterized the N-glycome of tsetse saliva glycoproteins. Tsetse salivary N-glycans were enzymatically released, tagged with either 2-aminobenzamide (2-AB) or procainamide, and analyzed by HILIC-UHPLC-FLR coupled online
with positive-ion ESI-LC-MS/MS. We found that the N-glycan profiles of T. brucei-infected and naïve tsetse salivary glycoproteins are almost identical, consisting mainly (>50%) of
highly processed Man3GlcNAc2 in addition to several other paucimannose, high mannose, and few hybrid-type N-glycans. In overlay assays, these sugars were differentially recognized
by the mannose receptor and DC-SIGN C-type lectins. We also show that salivary glycoproteins bind strongly to the surface of transmissible metacyclic trypanosomes. We suggest that although the repertoire of tsetse salivary N-glycans does not change during a trypanosome infection, the interactions with mannosylated glycoproteins may influence parasite
transmission into the vertebrate host.

Item Type:	Article
Subjects:	QU Biochemistry > Genetics > QU 460 Genomics. Proteomics QX Parasitology > QX 4 General works QX Parasitology > Insects. Other Parasites > QX 505 Diptera WC Communicable Diseases > Tropical and Parasitic Diseases > WC 705 Trypanosomiasis
Faculty: Department:	Biological Sciences > Vector Biology Department Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1371/journal.pntd.0009071
Depositing User:	Cathy Waldron
Date Deposited:	01 Mar 2021 13:29
Last Modified:	01 Mar 2021 13:29
URI:	https://archive.lstmed.ac.uk/id/eprint/17108

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