Negeviruses reduce replication of alphaviruses during co-infection

Patterson, Ian ORCID: https://orcid.org/0000-0003-3465-0848, Kautz, Tiffany F., Contreras-Gutierrez, Maria A., Guzman, Hilda, Tesh, Robert B., Hughes, Grant ORCID: https://orcid.org/0000-0002-7567-7185 and Forrester, Naomi L. (2021) 'Negeviruses reduce replication of alphaviruses during co-infection'. Journal of Virology, Vol 95, Issue 14, :e0043321.

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Abstract

Negeviruses are a group of insect-specific virus (ISV) that have been found in many arthropods. Their presence in important vector species led us to examine their interactions with arboviruses during co-infections. Wild-type negeviruses reduced the replication of several alphaviruses during co-infections in mosquito cells. Negev virus (NEGV) isolates were also used to express GFP and anti-chikungunya virus (CHIKV) antibody fragments during co-infections with CHIKV. NEGV expressing anti-CHIKV antibody fragments was able to further reduce replication of CHIKV during co-infections, while reductions of CHIKV with NEGV expressing GFP were similar to titers with wild-type NEGV alone. These results are the first to show that negeviruses induce superinfection exclusion of arboviruses and to demonstrate a novel approach to deliver anti-viral antibody fragments with paratransgenic ISVs. The ability to inhibit arbovirus replication and express exogenous proteins in mosquito cells make negeviruses a promising platform for control of arthropod-borne pathogens.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Viruses > QW 160 Viruses (General). Virology QW Microbiology and Immunology > Viruses > QW 162 Insect viruses QX Parasitology > Arthropods > QX 460 Arthropods
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1128/JVI.00433-21
Depositing User:	Mel Finley
Date Deposited:	13 May 2021 15:15
Last Modified:	05 Nov 2021 02:02
URI:	https://archive.lstmed.ac.uk/id/eprint/17813

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