Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children

Comella-Del-Barrio, Patricia, Izquierdo-Garcia, José Luis, Gautier, Jacqueline, Doresca, Mariette Jean Coute, Campos-Olivas, Ramón, Santiveri, Clara M, Muriel-Moreno, Beatriz, Prat-Aymerich, Cristina, Abellana, Rosa, Pérez-Porcuna, Tomas M, Cuevas, Luis ORCID: https://orcid.org/0000-0002-6581-0587, Ruiz-Cabello, Jesús and Domínguez, José (2021) 'Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children'. Scientific Reports, Vol 11, p. 12006.

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Abstract

Tuberculosis (TB) is a major cause of morbidity and mortality in children, and early diagnosis and treatment are crucial to reduce long-term morbidity and mortality. In this study, we explore whether urine nuclear magnetic resonance (NMR)-based metabolomics could be used to identify differences in the metabolic response of children with different diagnostic certainty of TB. We included 62 children with signs and symptoms of TB and 55 apparently healthy children. Six of the children with presumptive TB had bacteriologically confirmed TB, 52 children with unconfirmed TB, and 4 children with unlikely TB. Urine metabolic fingerprints were identified using high- and low-field proton NMR platforms and assessed with pattern recognition techniques such as principal components analysis and partial least squares discriminant analysis. We observed differences in the metabolic fingerprint of children with bacteriologically confirmed and unconfirmed TB compared to children with unlikely TB (p = 0.041 and p = 0.013, respectively). Moreover, children with unconfirmed TB with X-rays compatible with TB showed differences in the metabolic fingerprint compared to children with non-pathological X-rays (p = 0.009). Differences in the metabolic fingerprint in children with different diagnostic certainty of TB could contribute to a more accurate characterisation of TB in the paediatric population. The use of metabolomics could be useful to improve the prediction of TB progression and diagnosis in children.

Item Type:	Article
Subjects:	WF Respiratory System > Tuberculosis > WF 200 Tuberculosis (General) WF Respiratory System > Tuberculosis > WF 220 Diagnosis. Prognosis WS Pediatrics > Diseases of Children and Adolescents > By System > WS 280 Respiratory system
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1038/s41598-021-91545-0
Depositing User:	Julie Franco
Date Deposited:	05 Aug 2021 09:42
Last Modified:	05 Aug 2021 09:42
URI:	https://archive.lstmed.ac.uk/id/eprint/18572

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