Elevated activity of an Epsilon class glutathione transferase confers DDT resistance in the dengue vector, Aedes aegypti

Lumjuan, Nongkran, McCarroll, L., Prapanthadara, L. A., Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 and Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247 (2005) 'Elevated activity of an Epsilon class glutathione transferase confers DDT resistance in the dengue vector, Aedes aegypti'. Insect Biochemistry and Molecular Biology, Vol 35, Issue 8, pp. 861-871.

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Abstract

Glutathione transferases (GSTs) play a central role in the detoxification of xenobiotics such as insecticides and elevated GST expression is an important mechanism of insecticide resistance. In the mosquito, Anopheles gambiae, increased expression of an Epsilon class GST, GSTE2, confers resistance to DDT. We have identified eight GST genes in the dengue vector, Aedes aegypti. Four of these belong to the insect specific GST classes Delta and Epsilon and three are from the more ubiquitously distributed Theta and Sigma classes. The expression levels of the two Epsilon genes, a Theta GST and a previously identified Ae. aegypti GST [Grant and Hammock, 1992. Molecular and General Genetics 234, 169-176] were established for an insecticide susceptible and a resistant strain. We show that the putative ortholog of GSTe2 in Ae. aegypti (AaGSTe2) is over expressed in mosquitoes that are resistant to the insecticides DDT and permethrin. Characterisation of recombinant AaGSTE2-2 confirmed the role of this enzyme in DDT metabolism. In addition, unlike its Anopheles ortholog, AaGSTE2-2 also exhibited glutathione peroxidase activity. (c) 2005 Elsevier Ltd. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	glutathione transferase insecticide resistance aedes aegypti anopheles gambiae ddt dehydrochlorinase pyrethroids s-transferase insecticide-resistance anopheles-gambiae drosophila-melanogaster heterologous expression culex-quinquefasciatus nilaparvata-lugens molecular-cloning bac library mosquito
Subjects:	QU Biochemistry > Enzymes > QU 135 Enzymes QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 525 Aedes QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.ibmb.2005.03.008
Depositing User:	Martin Chapman
Date Deposited:	06 Sep 2011 13:25
Last Modified:	30 Aug 2019 17:05
URI:	https://archive.lstmed.ac.uk/id/eprint/1898

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