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Williams, Jessica 
ORCID: https://orcid.org/0000-0003-4416-5117, Ingham, Victoria ORCID: https://orcid.org/0000-0001-5708-4741, Morris, Marion, Toé, Kobié Hyacinthe, Hien, Aristide S., Morgan, John, Dabiré, Roch K., Guelbéogo, Wamdagogo Moussa, Sagnon, N’Falé and Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247
(2022)
'Sympatric Populations of the Anopheles gambiae Complex in Southwest Burkina Faso Evolve Multiple Diverse Resistance Mechanisms in Response to Intense Selection Pressure with Pyrethroids'. Insects, Vol 13, Issue 3, e247.
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Abstract
Pyrethroid resistance in the Anopheles vectors of malaria is driving an urgent search for new insecticides that can be used in proven vector control tools such as insecticide treated nets (ITNs). Screening for potential new insecticides requires access to stable colonies of the predominant vector species that contain the major pyrethroid resistance mechanisms circulating in wild populations. Southwest Burkina Faso is an apparent hotspot for the emergence of pyrethroid resistance in species of the Anopheles gambiae complex. We established stable colonies from larval collections across this region and characterised the resistance phenotype and underpinning genetic mechanisms. Three additional colonies were successfully established (1 An. coluzzii, 1 An. gambiae and 1 An. arabiensis) to add to the 2 An. coluzzii colonies already established from this region; all 5 strains are highly resistant to pyrethroids. Synergism assays found that piperonyl butoxide (PBO) exposure was unable to fully restore susceptibility although exposure to a commercial ITN containing PBO resulted in 100% mortality. All colonies contained resistant alleles of the voltage gated sodium channel but with differing proportions of alternative resistant haplotypes. RNAseq data confirmed the role of P450s, with CYP6P3 and CYP6Z2 elevated in all 5 strains, and identified many other resistance mechanisms, some found across strains, others unique to a particular species. These strains represent an important resource for insecticide discovery and provide further insights into the complex genetic changes driving pyrethroid resistance.
| Item Type: | Article |
|---|---|
| Additional Information: | This article belongs to the Special Issue Insecticides for Mosquito Control: Strengthening the Evidence Base |
| Subjects: | QX Parasitology > QX 20 Research (General) QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.3390/insects13030247 |
| SWORD Depositor: | JISC Pubrouter |
| Depositing User: | JISC Pubrouter |
| Date Deposited: | 29 Jun 2022 13:35 |
| Last Modified: | 15 Jun 2023 12:25 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/20052 |
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