The application of digital holography for accurate three-dimensional localisation of mosquito-bednet interaction

Hall, Matthew L, Gleave, Katherine, Hughes, Angela, McCall, Philip ORCID: https://orcid.org/0000-0002-0007-3985, Towers, Catherine E and Towers, David P (2022) 'The application of digital holography for accurate three-dimensional localisation of mosquito-bednet interaction'. Light: Advanced Manufacturing, Vol 3, Issue 1, e20.

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Abstract

Understanding mosquito interaction with long-lasting insecticidal bednets is crucial in the development of more effective intervention methods to protect humans from malaria transmission. As such, a 240 × 240 × 1000 mm laboratory setup for the in-line recording of digital holograms and subsequent in-focus reconstruction and 3D localisation of mosquitoes is presented. Simple bednet background removal methods are used to accurately localise a mosquito obscured by a bednet in 3D coordinates. Simulations and physical data demonstrate that this method is suitable for mosquitoes positioned 3−1000 mm behind a bednet. A novel post-processing technique, involving a cascade-correlation of a Tamura of Intensity focus metric extracted from digitally reconstructed scenes, accurately localises mosquitoes positioned 35−100 mm behind a bednet from a single digital hologram. The result of this study is a scalable digital holographic methodology to examine mosquito-bednet interaction in 3D at a level of accuracy previously only seen in 2D imaging of mosquitoes in a much smaller volume.

Item Type:	Article
Subjects:	QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) WN Radiology. Diagnostic imaging > WN 180 Diagnostic imaging (General)
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.37188/lam.2022.020
Depositing User:	Samantha Sheldrake
Date Deposited:	14 Mar 2022 12:44
Last Modified:	14 Mar 2022 12:44
URI:	https://archive.lstmed.ac.uk/id/eprint/20107

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