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Patterns of Kdr-L995F Allele Emergence Alongside Detoxifying Enzymes Associated with Deltamethrin Resistance in Anopheles gambiae s.l. from North Cameroon

Etang, Josiane, Mandeng, Stanislas Elysée, Nwane, Philippe, Awono-Ambene, Herman Parfait, Bigoga, Jude D, Ekoko, Wolfgang Eyisap, Binyang, Achille Jerome, Piameu, Michael, Mbakop, Lili Ranaise, Mvondo, Narcisse, Tabue, Raymond, Mimpfoundi, Rémy, Toto, Jean Claude, Kleinschmidt, Immo, Knox, Tessa Bellamy, Mnzava, Abraham Peter, Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497 and Fondjo, Etienne (2022) 'Patterns of Kdr-L995F Allele Emergence Alongside Detoxifying Enzymes Associated with Deltamethrin Resistance in Anopheles gambiae s.l. from North Cameroon'. Pathogens, Vol 11, Issue 2, e253.

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Abstract

Understanding how multiple insecticide resistance mechanisms occur in malaria vectors is essential for efficient vector control. This study aimed at assessing the evolution of metabolic mechanisms and Kdr L995F/S resistance alleles in Anopheles gambiae s.l. from North Cameroon, following long-lasting insecticidal nets (LLINs) distribution in 2011. Female An. gambiae s.l. emerging from larvae collected in Ouro-Housso/Kanadi, Be-Centre, and Bala in 2011 and 2015, were tested for susceptibility to deltamethrin + piperonyl butoxide (PBO) or SSS-tributyl-phosphoro-thrithioate (DEF) synergists, using the World Health Organization’s standard protocol. The Kdr L995F/S alleles were genotyped using Hot Ligation Oligonucleotide Assay. Tested mosquitoes identified using PCR-RFLP were composed of An. arabiensis (68.5%), An. coluzzii (25.5%) and An. gambiae (6%) species. From 2011 to 2015, metabolic resistance increased in Ouro-Housso/Kanadi (up to 89.5% mortality to deltametnrin+synergists in 2015 versus <65% in 2011; p < 0.02), while it decreased in Be-Centre and Bala (>95% mortality in 2011 versus 42–94% in 2015; p < 0.001). Conversely, the Kdr L995F allelic frequencies slightly decreased in Ouro-Housso/Kanadi (from 50% to 46%, p > 0.9), while significantly increasing in Be-Centre and Bala (from 0–13% to 18–36%, p < 0.02). These data revealed two evolutionary trends of deltamethrin resistance mechanisms; non-pyrethroid vector control tools should supplement LLINs in North Cameroon

Item Type: Article
Additional Information: This article belongs to the Special Issue Drug and Insecticide Resistances among Malaria Parasites and Vectors: Advances in Research and Mitigation Approaches
Subjects: QU Biochemistry > Enzymes > QU 135 Enzymes
QX Parasitology > QX 20 Research (General)
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.3390/pathogens11020253
SWORD Depositor: JISC Pubrouter
Depositing User: JISC Pubrouter
Date Deposited: 07 Jul 2022 11:00
Last Modified: 07 Jul 2022 11:00
URI: https://archive.lstmed.ac.uk/id/eprint/20188

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