How to sequence 10,000 bacterial genomes and retain your sanity: an accessible, efficient and global approach

Perez-Sepulveda, Blanca, Heavens, Darren, Pulford, Caisey, Predeus, Alexander, Low, Ross, Webster, Hermione, Dykes, Gregory, Schudoma, Christian, Rowe, Will, Lipscombe, James, Watkins, Chris, Kumwenda, Benjamin, Shearer, Neil, Costigan, Karl, Baker, Kate, Feasey, Nicholas ORCID: https://orcid.org/0000-0003-4041-1405, Hinton, Jay and Hall, Neil (2022) 'How to sequence 10,000 bacterial genomes and retain your sanity: an accessible, efficient and global approach' in Meeting.

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Abstract

Non-typhoidal Salmonella(NTS)are typically associated with enterocolitis and linked to the industrialisation of food production. In recent years, NTS has been associated with invasive disease (iNTS disease) causing an estimated 77,000 deaths each year worldwide; 80% of mortality occurs in sub-Saharan Africa. New clades of S. Typhimurium and S. Enteritidis have been identified, which are characterised by genomic degradation, altered prophage repertoires and novel multidrug resistant plasmids. To understand how these clades are contributing to the burden and severity of iNTS disease, it is crucial to expand genome-based surveillance to cover more countries, and incorporate historical isolates to generate an evolutionary timeline of the development of iNTS. We developedand validateda robust and inexpensive method for large-scale collection and sequencing of bacterial genomes. The “10,000 Salmonella genomes” project established a worldwide research collaboration to generate information relevant to the epidemiolog

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	Meeting report
Subjects:	QU Biochemistry > Genetics > QU 460 Genomics. Proteomics QW Microbiology and Immunology > QW 50 Bacteria (General). Bacteriology. Archaea
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1099/acmi.ac2021.po0349
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	24 Nov 2022 11:15
Last Modified:	22 Jan 2025 11:19
URI:	https://archive.lstmed.ac.uk/id/eprint/20527

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