Strain Characterisation for Measuring Bioefficacy of ITNs Treated with Two Active Ingredients (Dual-AI ITNs): Developing a Robust Protocol by Building Consensus

Lees, Rosemary ORCID: https://orcid.org/0000-0002-4232-9125, Armistead, Jennifer S., Azizi, Salum, Constant, Edi, Fornadel, Christen, Gimnig, John E., Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173, Impoinvil, Daniel, Irish, Seth R., Kisinza, William, Lissenden, Natalie ORCID: https://orcid.org/0000-0002-6578-5537, Mawejje, Henry D., Messenger, Louisa A., Moore, Sarah, Ngufor, Corine, Oxborough, Richard, Protopopoff, Natacha, Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247, Small, Graham, Wagman, Joseph, Weetman, David ORCID: https://orcid.org/0000-0002-5820-1388, Zohdy, Sarah and Spiers, Angus (2022) 'Strain Characterisation for Measuring Bioefficacy of ITNs Treated with Two Active Ingredients (Dual-AI ITNs): Developing a Robust Protocol by Building Consensus'. Insects, Vol 13, Issue 5, e434.

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Abstract

Durability monitoring of insecticide-treated nets (ITNs) containing a pyrethroid in combination with a second active ingredient (AI) must be adapted so that the insecticidal bioefficacy of each AI can be monitored independently. An effective way to do this is to measure rapid knock down of a pyrethroid-susceptible strain of mosquitoes to assess the bioefficacy of the pyrethroid component and to use a pyrethroid-resistant strain to measure the bioefficacy of the second ingredient. To allow robust comparison of results across tests within and between test facilities, and over time, protocols for bioefficacy testing must include either characterisation of the resistant strain, standardisation of the mosquitoes used for bioassays, or a combination of the two. Through a series of virtual meetings, key stakeholders and practitioners explored different approaches to achieving these goals. Via an iterative process we decided on the preferred approach and produced a protocol consisting of characterising mosquitoes used for bioefficacy testing before and after a round of bioassays, for example at each time point in a durability monitoring study. We present the final protocol and justify our approach to establishing a standard methodology for durability monitoring of ITNs containing pyrethroid and a second AI.

Item Type:	Article
Subjects:	QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
Faculty: Department:	Biological Sciences > Vector Biology Department IVCC
Digital Object Identifer (DOI):	https://doi.org/10.3390/insects13050434
Depositing User:	Georgia Harrison
Date Deposited:	24 Jun 2022 09:55
Last Modified:	24 Jun 2022 09:55
URI:	https://archive.lstmed.ac.uk/id/eprint/20603

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