Metabolic Processes Are Differentially Regulated During Wild-Type and Attenuated Dengue Virus Infection in Aedes aegypti

Siriphanitchakorn, Tanamas, Modahl, Cassandra, Manjunatha Kini, R., Ooi, Eng Eong and Choy, Milly (2022) 'Metabolic Processes Are Differentially Regulated During Wild-Type and Attenuated Dengue Virus Infection in Aedes aegypti'. American Journal of Tropical Medicine and Hygiene, Vol 106, Issue 3, pp. 900-904.

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Abstract

Successful completion of the dengue virus (DENV) life cycle in its mosquito vectors is important for efficient human–mosquito–human cycle of transmission, but the virus–mosquito interactions that underpin this critical event are poorly defined. To understand the virus–host interactions that determine viral infection by Aedes aegypti, the principal DENV vector, the authors compared transcriptomic changes in the head/thorax of the mosquito after intrathoracic infection with the wild-type DENV2 16681 strain and its attenuated derivative, PDK53. Using high-throughput RNA-sequencing, the authors identified 1,629 differentially expressed genes (DEGs) during 16681 infection, compared with only 22 DEGs identified during PDK53 infection, indicating that 16681 infection triggers a more robust host transcriptomic response compared with PDK53 infection. The authors further found that 16681 infection, but not PDK53 infection, altered metabolism in these heads/thoraces. Altogether, our findings reveal differential regulation of metabolic processes during wild-type and attenuated DENV infection, and suggest the need for future work to study the role of metabolic processes in determining DENV infection and replication in its mosquito vectors.

Item Type:	Article
Additional Information:	Short report
Uncontrolled Keywords:	NOT_LSTM
Subjects:	QX Parasitology > Insects. Other Parasites > QX 525 Aedes WC Communicable Diseases > Virus Diseases > Infectious Mononucleosis. Arbovirus Infections > WC 528 Dengue
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.4269/ajtmh.21-0734
Depositing User:	Clare Bennett
Date Deposited:	08 Mar 2023 09:42
Last Modified:	27 Jun 2023 10:15
URI:	https://archive.lstmed.ac.uk/id/eprint/21798

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